KHB720 - STEM CELL and GENE THERAPIES IN REGENERATIVE MEDICINE

Course Name Code Semester Theory
(hours/week)		 Application
(hours/week)		 Credit ECTS
STEM CELL and GENE THERAPIES IN REGENERATIVE MEDICINE KHB720 3rd Semester 3 0 3 9
PrequisitesNone
Course languageTurkish
Course typeElective 
Mode of DeliveryFace-to-Face 
Learning and teaching strategiesLecture
Discussion
Question and Answer
 
Instructor (s)Fatima AERTS KAYA (Coordinator), Research Assistants: AyÅŸen GÃœNEL ÖZCAN, Fatma Visal OKUR, Betül ÇELEBÄ° SALTIK, Duygu UÇKAN ÇETÄ°NKAYA 
Course objectiveThe aims of this course are; 1. To provide theoretical knowledge to the applicants on principals of regenerative medicine applications using stem cells and providing insights into novel applications and avenues for the implementation of new therapeutical ideas. 2. To provide theoretical knowledge to the applicants on principals of modification of cancer microenvironment, cancer cells and/or immune system to develop targeted therapies. Also laboratory methods used for this purpose will be explained in details in order to help them to gain the ability to discuss the results of current basic research and develop new projects that might be translated to clinic. 3. To introduce the concept of gene therapy, non integrative and integrative vector systems to the students. To discuss the different methods used for gene targeting and tracking. To teach the regulations and ethical rules of applicaions of gene targeting in clinic 
Learning outcomes
  1. Knowledge of the theoretical basis of regenerative medicine concepts, Discussing vausative effects and results of tissue and organ insufficiencies,
  2. The theoretical foundations of targeted therapy and discussing the cause and effect relationship in other targeted cellular therapies,
  3. The basics of gene therapy with hematopoietic and mesenchymal stem cells and knowledge of the different methods used in gene therapy,
  4. The advantages and disadvantages of nonintegrative and integrative vectors, Knowledge and prevention of immune reactions associated with gene therapy,
  5. Identifying possible risks and side effects of in vivo and ex vivo gene therapy. Designing a hypothetical gene therapy protocol that could target a specific tissue, disease or cell.
Course ContentDuring this course;
1. The principles of regenerative medicine and current clinical applications will be discussed with the participants accompanied by articles.
2. Oriented stem cell and regenerative medicine applications for repair of specific tissue and organ dysfunctions will be introduced.
3. Participants will be informed about tissue engineering applications used for regenerative medicine.
4. In vivo experimental applications in regenerative medicine will be introduced.
5. The general principles of laboratory and clinical practices in the light of basic concepts of targeted cellular therapy approaches will be transferred to participants in accompany with articles.
6. Concepts of gene therapy, gene replacement, gene silencing (with RNA interference and antisense technologies) and suicide gene therapy strategies will be discussed. 
References1. Stem Cells in Regenerative Medicine, Ed. Julie Audet and William L. Stanford, Humana Press, 2009 Toronto, Kanada.
2. Stem Cells and Human Diseases, Ed. Rakesh K. Srivastava, Sharmila Shankar, Springer Press, 2012, Heidelberg, London, New York.
3. Gene and Cell Therapy: Therapeutic Mechanisms, CRC press, 2009, editör: Templeton NS.
4. Immunology of gene therapy, Wiley-Blackwell 2008, editor: Herzog, R. ISBN13: 9780470134061.
5. A Guide To Human Gene Therapy, World Scientific Publishing Company 2010, editor: Herzog, R. ISBN13: 9789814280907.
6. Gianpietro Dotti, Barbara Savoldo, Fatma V. Okur, Raphael Rousseau, Malcolm K. Brenner (2008). Gene Therapy for the Treatment of Cancer: From Laboratory to Bedside. In Nancy Smyth Templeton (Editor), Gene and Cell Therapy: Therapeutic Mechanisms and Strategies (pp. 1001-1018). CRC Press. Stem Cells and Human Diseases, Ed. Rakesh K. Srivastara, Sharmila Shankar, Springer Press, 2012, Heidelberg, London, New York. 

Course outline weekly

WeeksTopics
Week 1Regenerative Medicine Concept (Ayşen GÜNEL-ÖZCAN, Duygu UÇKAN-ÇETİNKAYA)
Week 2Regeneration by Stem Cells (Duygu UÇKAN-ÇETİNKAYA)
Week 3Targeted Therapy Definition and Strategies (Fatma Visal OKUR)
Week 4Applications of Tissue Engineering (Betül ÇELEBİ-SALTIK)
Week 5In vivo Experimental Applications in Regenerative Medicine (Fatima AERTS-KAYA)
Week 6Discussion/Article (Application of Stem Cells in Regenerative Medicine) (Duygu UÇKAN-ÇETİNKAYA, Betül ÇELEBİ-SALTIK, Fatma Visal OKUR)
Week 7Midterm Exam
Week 8Definition and Strategies of Gene Therapy (Ayşen GÜNEL-ÖZCAN)
Week 9Nonintegrative Vector Systems in Gene Therapy (Ayşen GÜNEL-ÖZCAN)
Week 10Integrative Vector Systems in Gene Therapy (Fatima AERTS-KAYA)
Week 11Cell and Tissue Specific Gene Targeting and Tracking Methods (Fatima AERTS-KAYA)
Week 12Gene Therapy and Clinical Trials (Ayşen GÜNEL-ÖZCAN, Fatima AERTS-KAYA)
Week 13Legal Regulations and Ethical Rules in Regenerative Medicine Practices (Fatima AERTS-KAYA)
Week 14Discussion/Article (Tissue-Targeted Gene Therapy) (Ayşen GÜNEL-ÖZCAN)
Week 15Discussion/Article (Targeted Cellular Therapies) (Fatima AERTS-KAYA, Ayşen GÜNEL-ÖZCAN, Duygu UÇKAN-ÇETİNKAYA, Betül ÇELEBİ-SALTIK, Fatma Visal OKUR)
Week 16Final Exam

Assesment methods

Course activitiesNumberPercentage
Attendance14010
Laboratory00
Application00
Field activities00
Specific practical training00
Assignments00
Presentation115
Project00
Seminar00
Midterms125
Final exam150
Total100
Percentage of semester activities contributing grade succes230
Percentage of final exam contributing grade succes1570
Total100

WORKLOAD AND ECTS CALCULATION

Activities Number Duration (hour) Total Work Load
Course Duration (x14) 14 3 42
Laboratory 0 0 0
Application000
Specific practical training000
Field activities000
Study Hours Out of Class (Preliminary work, reinforcement, ect)148112
Presentation / Seminar Preparation11616
Project000
Homework assignment000
Midterms (Study duration)14040
Final Exam (Study duration) 16060
Total Workload31127270

Matrix Of The Course Learning Outcomes Versus Program Outcomes

D.9. Key Learning OutcomesContrubition level*
12345
1. They will know, understand, analyze and assess basic concepts/mechanisms of stem cells X   
2. Find hypothetical solution suggestions to questions/problems related to stem cell concepts and sciences X   
3. Find solutions to stem cell-related problems through hypothetical analyses and develop approaches for applications   X 
4. Understand, know stem cell procedures, plan, write and execute science-based projects   X 
5. Follow and apply developments related to the stem cells    X
6. Use state-of-the-art information technology produced in their line of work effectively     
7. Own knowledge and skills required to discuss stem cell sciences and related topics at an international level   X 
8. Discuss and defend opinions on theoretic and practical topics in a scientific environment   X 
9. Report and publish the results of their stem cell research     
10. Know ethical principles of stem cell research. Know its importance for individuals and society and respect ethical principles  X  
11. Inform people about topics related to stem cell sciences; know standards of stem cell laboratory applications, can apply biosafety rules   X 
12. Gain knowledge on stem cells and cellular treatments for clinical applications, use their knowledge in clinic-directed research   X 
13. Gain information and knowledge to work in the frame of the regulatory office and/or take responsibility for a stem cell/cellular treatment production-processing facility   X 

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest