KHB719 - BASICS of HEMATOPOIESIS and STEM CELL TRANSPLANTATION

Course Name Code Semester Theory
(hours/week)		 Application
(hours/week)		 Credit ECTS
BASICS of HEMATOPOIESIS and STEM CELL TRANSPLANTATION KHB719 2nd Semester 3 0 3 9
PrequisitesGoog reading skills in English are requed for papers
Course languageTurkish
Course typeElective 
Mode of DeliveryFace-to-Face 
Learning and teaching strategiesLecture
Discussion
Question and Answer
Observation
Field Trip
 
Instructor (s)Duygu UÇKAN-ÇETÄ°NKAYA (Coordinator); Teaching Assistant: Åžule ÃœNAL, Günay BALTA, Betül ÇELEBÄ°-SALTIK, Selin AYTAÇ, Fatma Visal OKUR, Fatima AERTS-KAYA; Barış KUÅžKONMAZ; AyÅŸen GÃœNEL ÖZCAN 
Course objectiveThe purpose of this class is to give the students with a scientific interest that may elicit the transfer of knowledge from the laboratory to the clinic by providing information on the principles of stem cell transplantation, experimental and clinical transplantation procedures, in addition to inform them on topics, such as good production standards for hematopoietic and mesenchymal stem cells that can be used in the clinics, quality control, legal regulations and ethical approaches. 
Learning outcomes
  1. The procedure of hematopoietic stem cell transfer and immunological mechanisms involved in transplantation,
  2. The sources of other adult stem cells, in vivo application of mesenchymal stem cells and mechanisms of healing,
  3. Information on the following topics: preparation regimes for stem cells applicable for clinical use, quality control tests, biobanking, as well as legal regulations and ethical approaches,
  4. In the light of the basic information obtained within the frame of the Program's other courses, evaluating the dynamics of in vivo applications and cellular interactions
Course ContentLectures will include
This course provides the principles of current clinical applications of transplantation, accompanied by scientific reading to the participants. The course consists of discussions with expert scientists, with experience in clinical application and quality management. Autologous, allogeneic and xenogeneic stem cell transplantation strategies, transplantation immunogenetics, stem cell trafficking, stem cell mobilization and homing mechanisms, hematopoietic stem cell transplantation complications will be explained and discussed.
Graft-versus-host and graft-versus-leukemia will be discussed, engraftment and reconstitution will be explained.
The students will observe the collection of bone marrow in the operating rooms and/or the collection of peripheral blood stem cells by using apheresis procedures 
References1.`Hematopoietic cell transplantation Fourth Edition, 2008, Editors: Appelbaum FR, Forman SJ et al, ISBN 978-1-4051-5348-5
2. http://www.stembook.org/
3. Thomas' Hematopoietic Cell Transplantation: Stem Cell Transplantation, Editor(s): Frederick R. Appelbaum, Stephen J. Forman, Robert S. Negrin, Karl G. Blume. Fourth Edition. 2009 Blackwell Publishing Ltd.
4. Stem Cell Transplantation. Anthony Dick Ho (Editor), Ronald Hoffman (Editor), Esmail D. Zanjani (Editor). 2006 Wiley-VCH, Verlog GmBH&Co. 

Course outline weekly

WeeksTopics
Week 1Intrauterine Hematopoesis (Åžule ÃœNAL)
Week 2Hematopoietic stem cell and postnatal hematopoiesis (Duygu UÇKAN-ÇETİNKAYA)
Week 3Molecular regulation of hematopoiesis; Hematopoietic microenvironment and niche (Günay BALTA, Betül ÇELEBİ-SALTIK)
Week 4Hereditary pathology of hematopoiesis (Selin AYTAÇ, Şule ÜNAL)
Week 5Hematologic malignancies and leukemic stem cells (Selin AYTAÇ, Fatma Visal OKUR)
Week 6Hematopoietic stem cell transplantation - animal models (Fatima AERTS KAYA)
Week 7Hematopoietic stem cell transplantation process, immunology and complications (Duygu UÇKAN-ÇETİNKAYA)
Week 8Midterm Exam
Week 9Hematopoietic stem cell processing and accreditation (Duygu UÇKAN-ÇETİNKAYA, Fatima Visal OKUR)
Week 10Hematopoietic stem cell transplantation in hereditary metabolic disorders and immunodeficiencies (Barış KUŞKONMAZ, Duygu UÇKAN-ÇETİNKAYA)
Week 11Hematopoietic stem cell gene therapy applications (Fatima AERTS-KAYA, Ayşen GÜNEL-ÖZCAN)
Week 12Non-hematopoietic stem cells and other cellular therapies (Barış KUŞKONMAZ)
Week 13Immune modulation with mesenchymal stem cells and hematopoietic stem cell transplantation (Ayşen GÜNEL-ÖZCAN Seminar
Week 14Cell/gene therapy regulations and ethical issues (Fatima AERTS KAYA)
Week 15Cellular therapy product development/accreditation (Duygu UÇKAN-ÇETİNKAYA)
Week 16Final Exam

Assesment methods

Course activitiesNumberPercentage
Attendance1410
Laboratory00
Application00
Field activities00
Specific practical training00
Assignments420
Presentation00
Project140
Seminar00
Midterms110
Final exam120
Total100
Percentage of semester activities contributing grade succes2080
Percentage of final exam contributing grade succes120
Total100

WORKLOAD AND ECTS CALCULATION

Activities Number Duration (hour) Total Work Load
Course Duration (x14) 14 3 42
Laboratory 0 0 0
Application000
Specific practical training000
Field activities000
Study Hours Out of Class (Preliminary work, reinforcement, ect)14456
Presentation / Seminar Preparation000
Project14343
Homework assignment22244
Midterms (Study duration)13535
Final Exam (Study duration) 15050
Total Workload33157270

Matrix Of The Course Learning Outcomes Versus Program Outcomes

D.9. Key Learning OutcomesContrubition level*
12345
1. They will know, understand, analyze and assess basic concepts/mechanisms of stem cells  X  
2. Find hypothetical solution suggestions to questions/problems related to stem cell concepts and sciences   X 
3. Find solutions to stem cell-related problems through hypothetical analyses and develop approaches for applications     
4. Understand, know stem cell procedures, plan, write and execute science-based projects     
5. Follow and apply developments related to the stem cells   X 
6. Use state-of-the-art information technology produced in their line of work effectively     
7. Own knowledge and skills required to discuss stem cell sciences and related topics at an international level   X 
8. Discuss and defend opinions on theoretic and practical topics in a scientific environment   X 
9. Report and publish the results of their stem cell research     
10. Know ethical principles of stem cell research. Know its importance for individuals and society and respect ethical principles    X
11. Inform people about topics related to stem cell sciences; know standards of stem cell laboratory applications, can apply biosafety rules    X
12. Gain knowledge on stem cells and cellular treatments for clinical applications, use their knowledge in clinic-directed research    X
13. Gain information and knowledge to work in the frame of the regulatory office and/or take responsibility for a stem cell/cellular treatment production-processing facility    X

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest