PBT618 - MASS SPECTROMETRY of MACROMOLECULES
Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
---|---|---|---|---|---|---|
MASS SPECTROMETRY of MACROMOLECULES | PBT618 | 2nd Semester | 3 | 0 | 3 | 8 |
Prequisites | ||||||
Course language | Turkish | |||||
Course type | Elective | |||||
Mode of Delivery | Face-to-Face | |||||
Learning and teaching strategies | Discussion Demonstration | |||||
Instructor (s) | Assoc. Prof. Dr. Ömür Çelikbıçak/ Prof. Dr. Bekir Salih | |||||
Course objective | Main objective of the course is to present some major concepts in the field of mass spectrometry and to demonstrate the vital importance and applications of mass spectrometry to determine chemical and physical properties of biomacromolecules (i.e., proteins, peptides and nucleotide chains) and synthetic polymers in today?s important interdisciplinary researches based on macromolecules. | |||||
Learning outcomes |
| |||||
Course Content | Principals of Mass Spectrometry. Ionization methods, mass analyzers and detectors. Gas phase ion molecule reactions, important thermochemical parameters and soft ionization methods can be used for analysis of macromolecules. Matrix?Assisted Laser Desorption/Ionization (MALDI) and Electrospray Ionization (ESI) Mass Spectrometry and their properties. Tandem Mass Spectrometry (MS/MS ve MSn) ve Multi?purpose hybride mass spectrometry techniques, MALDI and ESI mass spectrometry of synthetic polymersI (Molecular weight determination, molecular weight distribution, end group analysis ve PDI), Polymer mixtures and blends, copolymers and advanced polymer analysis using tandem mass spectrometry. Thermal analysis methods in mass spectrometry (Pyrolysis ve heated solid probe mass spectrometry). Mass spectrometry techniques used for detection of polymer?metal interactions (ICP?MS). | |||||
References | ? Hoffman E.d. and Stroobant, V., 2007, Mass Spectrometry: Principles and Applications, John Wiley&Sons Ltd., West Sussex, 489p. ? Gross, J., 2004, Mass Spectrometry; A textbook, Springer?Verlag Berlin Heidelberg, 518p. ? Dass, C., 2007, Fundamentals of contemporary mass spectrometry, John Wiley & Sons, Inc., Hoboken, New Jersey, 585p. ? Westman?Brinkmalm, A., and Brinkmalm, G., 2009, A mass spectrometer?s building blocks. Mass spectrometry: instrumentation, interpretation, and applications. Ekman, R., Silberring, J., Westman?Brinkmalm, A., Kraj, A., (eds.), Jonh Wiley&Sons Publication, Inc., New Jersey, pp. 3?105. ? Hillenkamp, F. and Karas, M., 2007, The MALDI Process and Method. MALDI MS: A Practical Guide to Instrumentation, Methods and Applications. Hillenkamp F., Peter? Katalini?, J., (eds.), WILEY?VCH Verlag GmbH & Co. KGaA, Weinheim, pp. 1?28. |
Course outline weekly
Weeks | Topics |
---|---|
Week 1 | Principals of Mass Spectrometry |
Week 2 | Ionization methods, mass analyzers and detectors. |
Week 3 | Gas phase ion molecule reactions, important thermochemical parameters and soft ionization methods can be used for analysis of macromolecules. |
Week 4 | Matrix?Assisted Laser Desorption/Ionization (MALDI) and Electrospray Ionization (ESI) Mass Spectrometry and their properties. |
Week 5 | Tandem Mass Spectrometry (MS/MS ve MSn) ve Multi?purpose hybride mass spectrometry techniques |
Week 6 | MALDI and ESI mass spectrometry of synthetic polymers?I (Molecular weight determination, molecular weight distribution, end group analysis ve PDI) |
Week 7 | Mid?term exam |
Week 8 | MALDI and ESI mass spectrometry of synthetic polymers?II (i.e., PS, PMMA, PVA, PIB etc.) |
Week 9 | MALDI and ESI mass spectrometry of synthetic polymers?III (Polymer mixtures and blends, copolymers, Advanced polymer analysis using tandem mass spectrometry) |
Week 10 | Thermal analysis methods in mass spectrometry (Pyrolysis ve heated solid probe mass spectrometry) and Mass spectrometry techniques used for detection of polymer?metal interactions (ICP?MS). |
Week 11 | Protein/peptide chemistry and analysis (Protein sequencing, proteolytic enzymes, systematic cleavages of proteins and peptides) MALDI and ESI mass spectrometry of protein and peptide mixtures. Mass spectrometric databases and their usage for protein |
Week 12 | Mass spectrometric analysis of nucleotide chains (i.e., DNA, RNA, oligonucleotides) |
Week 13 | Chromatography and Mass Spectrometry (LC?MS and GC?MS). Separation and purification methods can be used for synthetic and biological macromolecules prior to their mass spectrometric analysis. |
Week 14 | Analytical approach before macromolecular instrumental analysis and mass spectrometry |
Week 15 | Homework |
Week 16 | Final exam |
Assesment methods
Course activities | Number | Percentage |
---|---|---|
Attendance | 0 | 0 |
Laboratory | 0 | 0 |
Application | 0 | 0 |
Field activities | 0 | 0 |
Specific practical training | 0 | 0 |
Assignments | 2 | 20 |
Presentation | 0 | 0 |
Project | 0 | 0 |
Seminar | 0 | 0 |
Midterms | 1 | 30 |
Final exam | 1 | 50 |
Total | 100 | |
Percentage of semester activities contributing grade succes | 0 | 50 |
Percentage of final exam contributing grade succes | 0 | 50 |
Total | 100 |
WORKLOAD AND ECTS CALCULATION
Activities | Number | Duration (hour) | Total Work Load |
---|---|---|---|
Course Duration (x14) | 14 | 3 | 42 |
Laboratory | 0 | 0 | 0 |
Application | 0 | 0 | 0 |
Specific practical training | 0 | 0 | 0 |
Field activities | 0 | 0 | 0 |
Study Hours Out of Class (Preliminary work, reinforcement, ect) | 10 | 6 | 60 |
Presentation / Seminar Preparation | 0 | 0 | 0 |
Project | 0 | 0 | 0 |
Homework assignment | 1 | 70 | 70 |
Midterms (Study duration) | 1 | 30 | 30 |
Final Exam (Study duration) | 1 | 40 | 40 |
Total Workload | 27 | 149 | 242 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
D.9. Key Learning Outcomes | Contrubition level* | ||||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 |
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest