EFB730 - PHARM. BIOTECHNOLOGY RESEARCH TECH. & LAB METHODS
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| PHARM. BIOTECHNOLOGY RESEARCH TECH. & LAB METHODS | EFB730 | Any Semester/Year | 1 | 3 | 4 | 8 |
| Prequisites | EFB 720 | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Preparing and/or Presenting Reports Experiment Drill and Practice Other: Applied | |||||
| Instructor (s) | Prof. Dr. Betül ARICA YEGİN | |||||
| Course objective | The aim of this course titled `Pharmaceutical Biotechnology Research Techniques amd Laboratory Methods' is to present theoretical- practical knowledge and skills about the basic research techniques, laboratory methods, scientific reserach and ethical principles and good laboratory practices in the field of pharmaceutical biotechnology. | |||||
| Learning outcomes |
| |||||
| Course Content | Research techniques in pharmaceutical biotechnology, access to information, information technologies, databases, literature search, knowledge management, pharmaceutical biotechnology laboratory methods, safe working principles,biosafety, good laboratory practice,pharmaceutical biotechnology laboratory equipments (autoclaves, centrifuges, ultracentrifugation, electrophoresis devices, incubators, spectrophotometers, ELISA, imaging, and other) and applications, protein analysis, blotting, DNA analysis, pDNA enzyme analysis, purification and quantitation, the particle size analysis and microscopic analysis, anlysis in pharmacopoeias | |||||
| References | 1) Lecture Notes, 2. Databases 3. Equipments Technical Documents and Software Programs 4) Pharmacopoeias | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Research techniques in pharnaceutical biotechnology, scientific and ethical principles, information technologies, databases, literature search and access to information |
| Week 2 | Good laboratory practices in pharmaceutical biotechnology, laboratory safety, biosafety, waste disposal and environmental protection |
| Week 3 | Pharmaceutical biotechnology laboratory equipments |
| Week 4 | Pharmaceutical biotechnology laboratory equipments |
| Week 5 | Protein purification methods and quantitative analysis |
| Week 6 | Protein analysis and SDS- PAGE |
| Week 7 | DNA analysis and agarose gel electrophoresis |
| Week 8 | Blotting techniques and western blot |
| Week 9 | Bacterial culture and pDNA propagation |
| Week 10 | pDNA purification, restriction enyzme analysis, purity and quantitative analysis |
| Week 11 | Particle size and size distribution analysis in pharmaceutical biotechnology and applications of dynamic light scattering |
| Week 12 | Microscopic analysis in pharmaceutical biotechnology |
| Week 13 | Pharmacopeial analysis in pharmaceutical biotechnology and their applications |
| Week 14 | Evaluation of results, statistical analysis, report preparation and presentation and discussion |
| Week 15 | Assignment, seminar, presentation and discussion |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 14 | 0 |
| Laboratory | 12 | 10 |
| Application | 8 | 10 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 1 | 25 |
| Presentation | 1 | 10 |
| Project | 0 | 0 |
| Seminar | 1 | 20 |
| Midterms | 0 | 0 |
| Final exam | 1 | 25 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 37 | 75 |
| Percentage of final exam contributing grade succes | 1 | 25 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 1 | 14 |
| Laboratory | 10 | 4 | 40 |
| Application | 4 | 4 | 16 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 12 | 5 | 60 |
| Presentation / Seminar Preparation | 2 | 25 | 50 |
| Project | 0 | 0 | 0 |
| Homework assignment | 1 | 30 | 30 |
| Midterms (Study duration) | 0 | 0 | 0 |
| Final Exam (Study duration) | 1 | 30 | 30 |
| Total Workload | 44 | 99 | 240 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest