HÄ°S783 - FLUORESCENCE MICROSCOPES
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| FLUORESCENCE MICROSCOPES | HÄ°S783 | Any Semester/Year | 1 | 2 | 2 | 5 |
| Prequisites | - | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Observation Demonstration | |||||
| Instructor (s) | Prof. Pergin Atilla, MD, PhD., Prof. Dilara Zeybek, MD | |||||
| Course objective | Essential knowledge gain on fluorescence microscopy techniques | |||||
| Learning outcomes |
| |||||
| Course Content | - Flourescent dyes - Flourescent microscope types | |||||
| References | 1. Handbook of Biological Confocal Microscopy James Pawley Springer | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | - Working principles of fluorophores |
| Week 2 | - Fluorescence microscopy |
| Week 3 | - Wide-field fluorescence microscopy |
| Week 4 | - Confocal microscopy, Multiphoton microscopy |
| Week 5 | Midterm exam |
| Week 6 | - Introduction to super-resolution microscopy, new generation fluorophores |
| Week 7 | - STED (Stimulated Emmision Diplation) microscopy |
| Week 8 | - PALM/STORM microscopy, GSD (Ground State Depletion) microscopy |
| Week 9 | - Presentation |
| Week 10 | - TIRF (Total Internal Reflection) microscopy |
| Week 11 | - Live cell fluorescence microscopy |
| Week 12 | - 3D Light sheet microscopy |
| Week 13 | - CARS microscopy |
| Week 14 | - FLIM, FRAP, FRET, - CLEM (Corrolative Light and Electron Microscopy) |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 0 | 0 |
| Laboratory | 28 | 20 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 0 | 0 |
| Presentation | 1 | 5 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 1 | 25 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 30 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 1 | 14 |
| Laboratory | 14 | 2 | 28 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 4 | 56 |
| Presentation / Seminar Preparation | 1 | 12 | 12 |
| Project | 0 | 0 | 0 |
| Homework assignment | 0 | 0 | 0 |
| Midterms (Study duration) | 1 | 20 | 20 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 45 | 59 | 150 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Know the light and electron microscobic structure of the cells, tissues and organs; understanding the molecular and genetic components, establish the relationship between the structure function. | X | ||||
| 2. Understanding of the molecular mechanisms regulating human embryonic development , should be able to define the developmental disorders. | |||||
| 3. Know the basic techniques such as histochemistry, immunohistochemistry, electron microscopy, cell culture. | X | ||||
| 4. Must know and apply microscopic imaging techniques together and other quantitative methods such as stereology. | X | ||||
| 5. Must have the basic knowledge and competence necessary to perform laboratuary works such as IVF, stem cell technologies in cooperation with the clinics. | |||||
| 6. Must have knowledge, skills and attitudes that will contribute to education. | |||||
| 7. Should know how to obtain information to improve self and understand the importance of continuous learning. | X | ||||
| 8. Be able to put forward original hypotheses independently; plan and write original projects. | |||||
| 9. Have information and views on ethical issues on science and publication ethics and clinically related IVF and stem cell studies. | |||||
| 10. Be capable to participate in national and international scientific environments , make presentations and discussions. | |||||
| 11. Understand the evaluation criteria of the national and international journals and evaluate an article or project independently. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest