HÄ°S786 - TECHNIQUES of THE HISTOLOGICAL STUDY of THE HARD T
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| TECHNIQUES of THE HISTOLOGICAL STUDY of THE HARD T | HÄ°S786 | Any Semester/Year | 2 | 0 | 2 | 5 |
| Prequisites | ||||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Team/Group Work Preparing and/or Presenting Reports Problem Solving Brain Storming | |||||
| Instructor (s) | Prof. Petek Korkusuz, MD, PhD, Assist. Prof. Elif Karakoç, MD | |||||
| Course objective | To teach the histologic and immunohistologic processing and evaluation methods for hard tissues (bone and teeth) and associated semi hard tissues (cartialge, mensicus, tendon and ligament) in correlation with functional parameters is aimed. The experimental animal models including skeletal diseases and defect-repair models will be also covered related to the above mantioned protocols. | |||||
| Learning outcomes |
| |||||
| Course Content | The histologic structure and embryologic development of hard tissues (bone and teeth) and associated semi-hard tissues (cartilage, ligament, tendon, meniscus and fascia) will be teached at a basic level. The use laboratory animals following ethical regulations related to hard tissue applications will be discussed. Different experimental animal disease or injury models will be detailed including fracture, cartilage-bone defect models, arthritis and ligamnet-tendon repair models. The detailed protocols for sampling, fixation, embedding, staining, immunolabeling and microscopic screening of different hard and semi-hard tissues will be covered. The decalcified and undecalcified hard tissue processing and specific staining protocols for dental and skeletal tissues will be comperatively discussed. The essentials for evaluating skeletal and dental tissues under different types of light microscopes will be given. The basic knowledge for evaluating skeletal and dental tissues under scanning an | |||||
| References | 1. Human Embryology and Developmental Bıology. BM Carlson. 2. Developing Human: Clinically Oriented Embryology. KL Moore, TVN Persaud, MG Torchia. 3. Before We Are Born. KL Moore, TVN Persaud, MG, Torchia. 4. Langman's Medical Embryology. TW Sadler. 5. Histology: A Text and Atlas: MH Ross, W Pawlina. 6. Ten Cate's Oral Histology: Development, structure, and function. Ten Cate, Arnold Richard 7. Animal Models in Orthopaedic Research. YH An, RJ Friedman. 8. Histological Techniques. JB Bancroft M Gamble. 9. Bone morphometry. HE Takashi | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Bone and cartilage histology and embryology |
| Week 2 | Tendon, ligament, meniscus, fascia histology and embryology |
| Week 3 | Dental histology and embryology |
| Week 4 | The animal species and experimental animal models used for testing hard tissues |
| Week 5 | Fracture and bone defect models |
| Week 6 | Cartilage defect and arthritis models |
| Week 7 | Tendon, ligament, meniscus repair models |
| Week 8 | Dental repair models |
| Week 9 | Sampling and fixation of hard and semi-hard tissues |
| Week 10 | The decalcified and undecalcified protocols for hard and semi-hard tissues |
| Week 11 | Midterm exam |
| Week 12 | Processing, embedding and sectioning of hard and semi-hard tissues |
| Week 13 | Histochemical staining and immunohistochemical labeling of hard and semi-hard tissues |
| Week 14 | Histomorphometry, Light and electron microscopic evaluation |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 28 | 5 |
| Laboratory | 0 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 0 | 0 |
| Presentation | 1 | 10 |
| Project | 0 | 0 |
| Seminar | 2 | 10 |
| Midterms | 1 | 25 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 32 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 0 | 0 | 0 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 4 | 56 |
| Presentation / Seminar Preparation | 3 | 12 | 36 |
| Project | 0 | 0 | 0 |
| Homework assignment | 0 | 0 | 0 |
| Midterms (Study duration) | 1 | 10 | 10 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 33 | 48 | 150 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Know the light and electron microscobic structure of the cells, tissues and organs; understanding the molecular and genetic components, establish the relationship between the structure function. | X | ||||
| 2. Understanding of the molecular mechanisms regulating human embryonic development , should be able to define the developmental disorders. | |||||
| 3. Know the basic techniques such as histochemistry, immunohistochemistry, electron microscopy, cell culture. | X | ||||
| 4. Must know and apply microscopic imaging techniques together and other quantitative methods such as stereology. | X | ||||
| 5. Must have the basic knowledge and competence necessary to perform laboratuary works such as IVF, stem cell technologies in cooperation with the clinics. | |||||
| 6. Must have knowledge, skills and attitudes that will contribute to education. | |||||
| 7. Should know how to obtain information to improve self and understand the importance of continuous learning. | |||||
| 8. Be able to put forward original hypotheses independently; plan and write original projects. | |||||
| 9. Have information and views on ethical issues on science and publication ethics and clinically related IVF and stem cell studies. | |||||
| 10. Be capable to participate in national and international scientific environments , make presentations and discussions. | |||||
| 11. Understand the evaluation criteria of the national and international journals and evaluate an article or project independently. | |||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest