HÄ°S776 - CELLULAR DIFFERENTIATION, ADULT & EMBRIONIC STEM C
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| CELLULAR DIFFERENTIATION, ADULT & EMBRIONIC STEM C | HÄ°S776 | Any Semester/Year | 2 | 0 | 2 | 5 |
| Prequisites | - | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Team/Group Work Preparing and/or Presenting Reports Problem Solving | |||||
| Instructor (s) | Prof. Petek Korkusuz, MD, PhD., Prof. Dilara Zeybek, MD., Prof. Lale Karakoç Sökmensüer, MD. | |||||
| Course objective | Evaluation of the differentiation stages and the structural-functional regulatory mechanisms of the 200 different types of human cells on their way to gain highly specific functions. Have the ability to discuss the features, potentials and functions of embryonal and adult (mesenchymal and hematopoetic) stem cells, tissue specific progenitor cells and inducible pluripotent stem cells. Gain skills in: fertilization by assisted reproductive techniques, embryo culture and transfer, cryopreservation of gamete-embryo, the use of embryonic cells in diagnosis and scientific studies. | |||||
| Learning outcomes |
| |||||
| Course Content | - The tissue specific differentiation of cells owing the same genetic material but different genetic expressions under the influence of various microenvironmental signals are discussed. - The sources, capacities, molecular and functional features of the embryonal and adult (mesenchymal, hematopoetic, IPS) stem cells with multilineage differentiation ability, are detailed with their therapeutic uses and future perspectives. - The embryo development, culture and, quality evaluation will be detailed in addition to the cryopreservation. - The use of embryonic and adult stem cells in clinical and scientific studies are discussed including ethical perspectives | |||||
| References | 1. Lanza ed.: Essentials of Stem Cell Biology, 2. Albert B., Johnson A eds.: Molecular Biology of The Cell. 3. Zon ed.: Current Protocols in Stem Cell Biology 4. Vemuri, Chase and Rao eds; Mesenchymal Stem Cell Assays and Applications 5. Ross MH, Kaye GI, Pawlina W: Histology. A Text and Atlas. 6. Carlson BM ed: Human Embryology and Developmental Biology. 7. Moore KL, Persaud TVN: The Developing Human. Clinically Oriented Embryology. 8. Gardner DK: Textbook of Assisted Reproductive Techniques. 9. Trounson A. and Gosden R: Biology and Pathology of the Oocyte 10. Veek L : An Atlas of Human Blastocysts, The Parthenon Publishing Group | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Cell cycle, proliferation and differentiation |
| Week 2 | Cell death, somatic and germ cells in their microenvironment |
| Week 3 | Spermatogenezis and oogenezis |
| Week 4 | Definition, classification and basic properties of stem cells |
| Week 5 | Embrionic stem cells, primordial germ cells |
| Week 6 | Mesenchymal stem cells |
| Week 7 | Hematopoetic stem cells |
| Week 8 | Induced pluripotent stem cells |
| Week 9 | Molecular mechanisms in stem cell programming |
| Week 10 | Midterm exam |
| Week 11 | Sperm maturation, sperm capacitation, acrosomal reaction, gamet interaction, oocyte activation |
| Week 12 | Evaluation of fertilization, molecular mechanisms of fertilization, failure of fertilization, in vivo and in vitro embryo development, quality evaluation of the embryo, embryo-scoring, |
| Week 13 | Preimplantation genetical diagnosis, embryo culture, biopsy of embryo blastomer and polar body, embryo culture systems, and co-culture, nuclear and cytoplasmic properties of human embryos, blastocyte development and transfer |
| Week 14 | Basic principles in cryopreservation: cryopreservation of oocyte and ovarian tissue, protection of fertility, cryopreservation of embryo, spermatozoa and testicular tissue |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 28 | 5 |
| Laboratory | 0 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 0 | 0 |
| Presentation | 1 | 10 |
| Project | 0 | 0 |
| Seminar | 1 | 10 |
| Midterms | 1 | 25 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 31 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 0 | 0 | 0 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 5 | 70 |
| Presentation / Seminar Preparation | 2 | 6 | 12 |
| Project | 0 | 0 | 0 |
| Homework assignment | 0 | 0 | 0 |
| Midterms (Study duration) | 1 | 20 | 20 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 32 | 53 | 150 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Know the light and electron microscobic structure of the cells, tissues and organs; understanding the molecular and genetic components, establish the relationship between the structure function. | |||||
| 2. Understanding of the molecular mechanisms regulating human embryonic development , should be able to define the developmental disorders. | |||||
| 3. Know the basic techniques such as histochemistry, immunohistochemistry, electron microscopy, cell culture. | |||||
| 4. Must know and apply microscopic imaging techniques together and other quantitative methods such as stereology. | |||||
| 5. Must have the basic knowledge and competence necessary to perform laboratuary works such as IVF, stem cell technologies in cooperation with the clinics. | X | ||||
| 6. Must have knowledge, skills and attitudes that will contribute to education. | |||||
| 7. Should know how to obtain information to improve self and understand the importance of continuous learning. | X | ||||
| 8. Be able to put forward original hypotheses independently; plan and write original projects. | X | ||||
| 9. Have information and views on ethical issues on science and publication ethics and clinically related IVF and stem cell studies. | X | ||||
| 10. Be capable to participate in national and international scientific environments , make presentations and discussions. | X | ||||
| 11. Understand the evaluation criteria of the national and international journals and evaluate an article or project independently. | |||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest