HÄ°S792 - IMMUNOHISTOCHEMISTRY TECHNIQUES
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| IMMUNOHISTOCHEMISTRY TECHNIQUES | HÄ°S792 | Any Semester/Year | 1 | 2 | 2 | 5 |
| Prequisites | ||||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Other | |||||
| Instructor (s) | Prof. Petek Korkusuz, MD, PhD., Prof. Pergin Atilla, MD, PhD., Assist. Prof. Elif Karakoç, MD | |||||
| Course objective | Learning principles of immunohistochemistry, achieving knowledge and skill to perform immunohistochemical examination | |||||
| Learning outcomes |
| |||||
| Course Content | 1. Discuss basic principles of immunohistochemistry and to determine the antibodies to be used for the research planned 2. To determine the best fixation technique according to the antigen to be demonstrated (soft tissues, blood smears, culture cells etc.) 3. Performing immunostaining both on frozen and paraffin embedded samples 4. To determine the suitable chromogen 5. To examine the immunostained techniques under microscope 6. To evaluate the reaction on sections 7. To perform Double-labeling techniques 8. To perform Pre- and post-embedding techniques at EM level 9. To perform immunogold technique | |||||
| References | 1) Hayat MA. Microscopy, Immunohistochemistry, and Antigen Retrieval Methods, 2) Kiernan JA. Histological & Histochemical Methods, Theory & Practice 3) Beltz BS, Burd GD. Immunocytochemical techniques, Principles & Practice | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Basics of immunohistochemical techniques and terminology |
| Week 2 | Fixation and buffers |
| Week 3 | Antibodies in immunohistochemistry |
| Week 4 | Antigen retrieval and amplification of signals |
| Week 5 | Blocking methods |
| Week 6 | Midterm exam |
| Week 7 | Enzyme based immunohistochemical techniques |
| Week 8 | Enzyme based immunohistochemical techniques- Practice |
| Week 9 | Immunfloresance hidtochemistry techniques |
| Week 10 | Immunfloresance hidtochemistry techniques: Practice |
| Week 11 | Basics of immunoelectronmicroscopic techniques: pre-embedding, post-embedding and Tokuyasu techniques |
| Week 12 | Basics of immunoelectronmicroscopic techniques:Practice |
| Week 13 | Evaluation and interpretation of immunlabeled samples at light microscopic level |
| Week 14 | Evaluation and interpretation of immunelectronmicroscopic samples |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 14 | 5 |
| Laboratory | 28 | 5 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 1 | 5 |
| Presentation | 1 | 5 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 1 | 30 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 45 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 1 | 14 |
| Laboratory | 14 | 2 | 28 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 4 | 56 |
| Presentation / Seminar Preparation | 1 | 6 | 6 |
| Project | 0 | 0 | 0 |
| Homework assignment | 1 | 6 | 6 |
| Midterms (Study duration) | 1 | 20 | 20 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 46 | 59 | 150 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest