HÄ°S753 - LABORATORY TECHNIQUES IN LIGHT MICROSCOPY & MICROS
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| LABORATORY TECHNIQUES IN LIGHT MICROSCOPY & MICROS | HÄ°S753 | 1st Semester | 1 | 2 | 2 | 5 |
| Prequisites | ||||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Team/Group Work Preparing and/or Presenting Reports Problem Solving | |||||
| Instructor (s) | Prof. Sevda Müftüoğlu, MD, PhD, Prof. Petek Korkusuz, MD, PhD, Prof. Dilara Zeybek, MD | |||||
| Course objective | Having knowledge about light microscopy techniques for tissues and organs, their processing and preparation | |||||
| Learning outcomes |
| |||||
| Course Content | 1) Fixation methods for soft and hard tissues; 2) Theory and practice of tissue processing 3)Microtomy on paraffin wax-embedded or frozen tissues 4)Theory ofstaining and its practical implications according to special compartments of the cell or tissue 5) Histochemical methods for special molecules 6) Mounting and examination of the slides under the light microscope | |||||
| References | 1) Theory and Practice of Histological Techniques. Edited by J.D. Bancroft, Marilyn Gamble . | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Description of the Histological Chemicals and equipments used in Light microscopy Laboratory |
| Week 2 | Fixation and fixatives |
| Week 3 | Tissue processing methods |
| Week 4 | Tissue processing and microtomy |
| Week 5 | Theory of staining and its practical implications. The Hematoxylin and Eosin staining |
| Week 6 | Midterm exam |
| Week 7 | Connective tissues and stainings |
| Week 8 | Special staining methods for lipids |
| Week 9 | Special staining methods for carbohydrates |
| Week 10 | Special staining methods for proteins and nucleic acids |
| Week 11 | Midterm exam |
| Week 12 | Techniques for the hard tissues (Bone, tooth etc) |
| Week 13 | Mounting and mounting mediums |
| Week 14 | Cryo-fixation and cryotomy; staining of the cryosections, Application of microwave technology to histology |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 14 | 5 |
| Laboratory | 28 | 5 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 1 | 5 |
| Presentation | 1 | 5 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 1 | 30 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 45 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 1 | 14 |
| Laboratory | 14 | 2 | 28 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 4 | 56 |
| Presentation / Seminar Preparation | 1 | 6 | 6 |
| Project | 0 | 0 | 0 |
| Homework assignment | 1 | 6 | 6 |
| Midterms (Study duration) | 1 | 20 | 20 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 46 | 59 | 150 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Accesses medical literature on basic and clinical immunology and -related fields and evaluates them. | X | ||||
| 2. Gains the necessary theoretical knowledge for their research and formulates hypotheses. Conducts research on immune system components and molecular structures, plans the appropriate methodology. | X | ||||
| 3. Investigates gene structures from molecular literature studies, demonstrates them through sequence analysis, interprets the data, reaches conclusions, and publishes these findings. | X | ||||
| 4. Demonstrates comprehensive and systematic knowledge on basic immunology, including understanding immunological mechanisms, immune system components, and their functions, also possesses knowledge in molecular genetics and biology. | X | ||||
| 5. Utilizes current statistical approach to evaluate the obtained data. Presents research at national and international conferences and publishes findings under mentorship. | X | ||||
| 6. Analyzes published immunological findings, proposes timely and effective solutions to encountered challenges in the laboratory, or communicates with researchers for solutions. | X | ||||
| 7. Upon completion of the basic immunology master's program, the student, under the guidance of their consultant, drafts a research proposal adhering to ethical guidelines, determines the budget, provides feedback on projects authored by other researchers, participates in collaborative projects from the planning stage, offers insights, suggests changes, and presents alternative solutions. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest