HÄ°S772 - CELL IN MICROSCOPIC and MOLECULAR LEVEL
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| CELL IN MICROSCOPIC and MOLECULAR LEVEL | HÄ°S772 | 1st Semester | 2 | 0 | 2 | 5 |
| Prequisites | - | |||||
| Course language | Turkish | |||||
| Course type | Must | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Other | |||||
| Instructor (s) | Prof. Sevda Müftüoğlu, MD, PhD., Prof. Dilara Zeybek MD., Assis. Prof. Elif Karakoç, MD | |||||
| Course objective | To learn the cell structure with all of its components, including cell membrane, organelles , nucleus and nucleolus at the light , electron microscopic and molecular levels and to define the relations of the cells with each other and extracellular matrix components. To discuss the fine structural and molecular criteria of cell signaling, cell migration, cell division and differentiation under the light of the latest literature. | |||||
| Learning outcomes |
| |||||
| Course Content | The basic structure of the cell including cell membrane, mitochondria, ER, lysosomes, endosomes, intracellular vesicles; nucleus and nucleolus and their role in the control of various cellular activities; structure of the cytoskeleton and its relation with the extracellular matrix components will be learned. Cell cycle, phases of cell division, maturation and cell death will also be evaluated at structural/molecular level. | |||||
| References | 1) Ross MH, Kaye GI, Pawlina W: Histology. A Text and Atlas, Williams & Wilkins, Baltimore. 2) Stevens A, Lowe J: Histology. Mosby-Year Book Europe Limited, London 3) Fawcett DW, Jensh RP, Bloom W: Bloom and Fawcett a Textbook of Histology, Lippincott Williams & Wilkins Publishers, Philadelphia, 4) Cooper GM.: The Cell, A Molecular Approach. 5) Albert B., Johnson A et al.: Molecular Biology of The Cell 6) Gartner L P, Hiatt J L : Histology, WB Saunders Co, Philadelphia, | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Description of cell, its components, cell membrane, molecular and fine structural properties; Transport through the cell membrane ; structures and mechanisms related to trans membrane transport |
| Week 2 | Protein synthesis : ribosome, GER . Synthesis of cytosolic and membrane bound proteins at light, electron microscobic and molecular levels. |
| Week 3 | Light and EM structure of the Golgi complex; Function/structure relations: maturation of proteins in Golgi complex, molecules that participates in this process. GER-Golgi relations; trans-Golgi network, protein sorting and transport, constitutive an |
| Week 4 | Mitochondrion; light and electron microscobic structure, Examples and functions of mitochondria rich cells in human. Structural variations /alterations in mitochondria related to its functional competence. Genetic inheritance, the importance of the b |
| Week 5 | Peroxisomes and other non-membranous organelles and inclusions . |
| Week 6 | Nucleus; DNA, RNA, gene, gene expression, flow of the genetic information and their structural indicators |
| Week 7 | Nucleolus; rRNA, ribosome formation, its transport |
| Week 8 | Presentation, Cell cycle cell division Programmed cell death and other types of cell death |
| Week 9 | Midterm exam |
| Week 10 | Cytoskeleton and its basic components: microtubules, microfilaments, intermediate filaments; properties and their demonstrations at the microscopic level |
| Week 11 | Junctional complexes; their structure and function- cell surface molecules related to junctions, integrins, cadherins, claudins, desmogleins Apical cell membrane specialisations; structure and relations with the cytoskeleton |
| Week 12 | Cell-extracellular matrix relations; their structure and function. Components of the extracellular matrix-GAG, proteoglycans, glycoproteins and fibrils |
| Week 13 | Molecular structure and function of basal lamina; definition of its structural alterations in various pathologies. |
| Week 14 | Cell adhesion molecules, extracellular matrix receptors, integrinler and functions of other cell membrane proteins in intracellular signaling mechanisms |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 28 | 10 |
| Laboratory | 0 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 1 | 5 |
| Presentation | 1 | 5 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 1 | 30 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 31 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 0 | 0 | 0 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 5 | 70 |
| Presentation / Seminar Preparation | 1 | 12 | 12 |
| Project | 0 | 0 | 0 |
| Homework assignment | 1 | 10 | 10 |
| Midterms (Study duration) | 1 | 10 | 10 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 32 | 59 | 150 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Know the light and electron microscobic structure of the cells, tissues and organs; understanding the molecular and genetic components, establish the relationship between the structure function. | X | ||||
| 2. Understanding of the molecular mechanisms regulating human embryonic development , should be able to define the developmental disorders. | X | ||||
| 3. Know the basic techniques such as histochemistry, immunohistochemistry, electron microscopy, cell culture. | |||||
| 4. Must know and apply microscopic imaging techniques together and other quantitative methods such as stereology. | |||||
| 5. Must have the basic knowledge and competence necessary to perform laboratuary works such as IVF, stem cell technologies in cooperation with the clinics. | X | ||||
| 6. Must have knowledge, skills and attitudes that will contribute to education. | X | ||||
| 7. Should know how to obtain information to improve self and understand the importance of continuous learning. | X | ||||
| 8. Be able to put forward original hypotheses independently; plan and write original projects. | X | ||||
| 9. Have information and views on ethical issues on science and publication ethics and clinically related IVF and stem cell studies. | X | ||||
| 10. Be capable to participate in national and international scientific environments , make presentations and discussions. | X | ||||
| 11. Understand the evaluation criteria of the national and international journals and evaluate an article or project independently. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest