SBT686 - LABORATORY METHODS IN MUSCLE PHYSIOLOGY II
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| LABORATORY METHODS IN MUSCLE PHYSIOLOGY II | SBT686 | 2nd Semester | 2 | 2 | 4 | 10 |
| Prequisites | SBT685 Laboratory Methods in Muscle Physiology I | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Experiment Project Design/Management | |||||
| Instructor (s) | Haydar A. Demirel, M.D., Ph.D. | |||||
| Course objective | This course is designed to give student, who works on muscle physiology, some laboratory techniques of muscle physiology, biochemistry and molecular biology before starting on working her/his own project. | |||||
| Learning outcomes |
| |||||
| Course Content | This course consists of both lectures and laboratory application. Basically, lectures will focus on therotical bacgrounds for the laboratory sections. Skeletal and cardiac muscle phenotype, stress response to skeletal muscle such as acute and chronic exercises, heat stress, hypoxia will be discussed. In that respect, both SDS poliacrylamide and native tube gel electrophoresis, western and immunohistochemical analysis of stress markers, antioxidant and anaerobic/aerobic enzyme activities along with the levels of oxidative muscle damage marker will be analyzed in the laboratory. | |||||
| References | There will be no suggested textbook. On the other hand, methods parts of various papers will be used as a course material. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | General principles of SDS gel electrophoresis |
| Week 2 | Prepareation of muscle samples for SDS gel electrophoresis |
| Week 3 | Isolation of skeletal muscle MHC?s by using SDS gel electrophoresis. |
| Week 4 | Isolation of skeletal muscle MLC?sby using SDS gel electrophoresis |
| Week 5 | Isolation of cardiac muscle myosin by using native gel electrophoresis |
| Week 6 | Assessment of muscle phenotype |
| Week 7 | Various stress models: Exercise training, hypoxia, heat |
| Week 8 | Immunohistochemistry |
| Week 9 | Western analysis |
| Week 10 | Western analysis |
| Week 11 | Measurement of citrate synthase enzyme activity |
| Week 12 | Measurement of myofibrillar ATPase activity |
| Week 13 | Measurement of SOD/GPx activities |
| Week 14 | Measurement of protein carbonyl in muscle tissue; Measurement of lipid peroxidation in muscle tissue |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 14 | 0 |
| Laboratory | 14 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 0 | 0 |
| Presentation | 0 | 0 |
| Project | 1 | 30 |
| Seminar | 2 | 20 |
| Midterms | 0 | 0 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 16 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 14 | 2 | 28 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 10 | 140 |
| Presentation / Seminar Preparation | 2 | 12 | 24 |
| Project | 1 | 30 | 30 |
| Homework assignment | 0 | 0 | 0 |
| Midterms (Study duration) | 0 | 0 | 0 |
| Final Exam (Study duration) | 1 | 50 | 50 |
| Total Workload | 46 | 106 | 300 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Develops and enhances expertise in movement and sport based on undergraduate competences. | X | ||||
| 2. Possesses necessary technological knowledge in exercise and sport science. | X | ||||
| 3. Understands research methodology in exercise and sport science. | X | ||||
| 4. Applies theoretical and practical knowledge effectively in exercise and sport science. | X | ||||
| 5. Synthesizes information from various fields to develop new analysis, synthesis, and solutions in exercise and sport sciences. | X | ||||
| 6. Plans, conducts, and reports scientific research in exercise and sport sciences. | X | ||||
| 7. Utilizes technological equipment to solve problems in exercise and sport science. | X | ||||
| 8. Develops and evaluates national/international strategies and policies in exercise and sport. | X | ||||
| 9. Works independently or as part of a team in exercise and sport sciences. | X | ||||
| 10. Publishes scientific articles or presents papers in national journals or scientific meetings. | X | ||||
| 11. Embraces lifelong learning, critically analyzing information in exercise and sport sciences. | X | ||||
| 12. Reads, analyzes, and conducts evidence-based research in exercise and sport science. | X | ||||
| 13. Critically analyzes and evaluates professional social environment norms and values. | X | ||||
| 14. Proficient in at least one European language at B2 level. | X | ||||
| 15. Assimilates, evaluates, and communicates variables and data in Exercise and Sport Science, considering relevant social, scientific, and ethical principles. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest