SBT685 - LABORATORY METHODS IN MUSCLE PHYSIOLOGY I
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| LABORATORY METHODS IN MUSCLE PHYSIOLOGY I | SBT685 | 1st Semester | 2 | 2 | 4 | 10 |
| Prequisites | None | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Experiment Project Design/Management | |||||
| Instructor (s) | Haydar A. Demirel, M.D., Ph.D. | |||||
| Course objective | This course is designed to give student, who works on muscle physiology, basic principles and the methodology of muscle physiology and biochemistry laboratory techniques before starting on working her/his own project. | |||||
| Learning outcomes |
| |||||
| Course Content | This course consists of both lectures and laboratory application. Basically, lectures will focus on therotical bacgrounds for the laboratory sections. In that respect, skeletal muscle structure, localization and characteristics, histochemically fiber typing, acut and chronic exercise tranining models via tradmill running and swimming, muscle atrophy models, preperation of basic chemical solutions and various methods to measure muscle protein content will be discussed and applied in the laboratory. | |||||
| References | There will be no suggested textbook. On the other hand, methods parts of various papers will be used as a course material. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Exercise training: Treadmill running |
| Week 2 | Exercise training: Swimming |
| Week 3 | Muscle atrophy models: Hindlimb suspension |
| Week 4 | Macroscopic apperance of skeletal muscles: White and Red muscles and their localizations |
| Week 5 | How to identify and isolate various locomotor muscles and left and right ventricle |
| Week 6 | Preperation of muscle sections for both histochemistry and biochemical measurement |
| Week 7 | Histochemistry for fiber typing: Cutting muscle sections by using cryostat |
| Week 8 | Preparing solutions for ATPase histochemistry for skeletal muscle fiber typing |
| Week 9 | Fiber type determination, measuring CSA, assessment of fiber type distribution |
| Week 10 | Paper discussion related to histochemical fiber typing |
| Week 11 | Preperations of various biochemical solutions, homogenization of muscle tissue |
| Week 12 | Isolation of myofibrillar and connective tissue protein |
| Week 13 | How to determine muscle protein content: Biuret, Bradford and Lowry methods |
| Week 14 | Measurement of myofibrillar and connective tissue protein |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 14 | 0 |
| Laboratory | 14 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 0 | 0 |
| Presentation | 0 | 0 |
| Project | 1 | 30 |
| Seminar | 2 | 20 |
| Midterms | 0 | 0 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 16 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 14 | 2 | 28 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 10 | 140 |
| Presentation / Seminar Preparation | 2 | 12 | 24 |
| Project | 1 | 30 | 30 |
| Homework assignment | 0 | 0 | 0 |
| Midterms (Study duration) | 0 | 0 | 0 |
| Final Exam (Study duration) | 1 | 50 | 50 |
| Total Workload | 46 | 106 | 300 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Develops and enhances expertise in movement and sport based on undergraduate competences. | X | ||||
| 2. Possesses necessary technological knowledge in exercise and sport science. | X | ||||
| 3. Understands research methodology in exercise and sport science. | X | ||||
| 4. Applies theoretical and practical knowledge effectively in exercise and sport science. | X | ||||
| 5. Synthesizes information from various fields to develop new analysis, synthesis, and solutions in exercise and sport sciences. | X | ||||
| 6. Plans, conducts, and reports scientific research in exercise and sport sciences. | X | ||||
| 7. Utilizes technological equipment to solve problems in exercise and sport science. | X | ||||
| 8. Develops and evaluates national/international strategies and policies in exercise and sport. | X | ||||
| 9. Works independently or as part of a team in exercise and sport sciences. | X | ||||
| 10. Publishes scientific articles or presents papers in national journals or scientific meetings. | X | ||||
| 11. Embraces lifelong learning, critically analyzing information in exercise and sport sciences. | X | ||||
| 12. Reads, analyzes, and conducts evidence-based research in exercise and sport science. | X | ||||
| 13. Critically analyzes and evaluates professional social environment norms and values. | X | ||||
| 14. Proficient in at least one European language at B2 level. | X | ||||
| 15. Assimilates, evaluates, and communicates variables and data in Exercise and Sport Science, considering relevant social, scientific, and ethical principles. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest