BYL682 - TECHNICAL PREPARATION

Course Name Code Semester Theory
(hours/week)
Application
(hours/week)
Credit ECTS
TECHNICAL PREPARATION BYL682 1st Semester 1 4 2 9
Prequisites
Course languageTurkish
Course typeElective 
Mode of DeliveryFace-to-Face 
Learning and teaching strategiesLecture
Discussion
Question and Answer
Demonstration
 
Instructor (s)Prof. Dr. Nurhayat Barlas 
Course objectiveExamining light microscopic section Planes of tissues Paraffin method for light microscopy, Tissue procurement, Fixation, Dehydration, Clearing, Embedding, Sectioning, Staining and Investigation  
Learning outcomes
  1. At the end of this course, The student learns staining, embedding, sectioning, preparation technique and examination methods which is taking tissue from laboratory animals Learns experiments associated with laboratory studies
Course ContentThe usage of microscopes, instruments and matters
The fixation of various cells and tissues of animals
Many fixation materials
Sectioning,
General and special staining
Immunohistochemistry
Prepara¬tion of museum materials.
The special methods for the preservation of museum materials
Cryıotomy
EM techniques
 
ReferencesIntroduction of Functional Histology, Ira R.Telford and Charles F. Brıdgman. Harper Collins College Publishers, 10East 53rd Street, New York.
Basic Histology, Carlos Junqueria, Jose Carneiro, Robert O. Kelly. Prentice-Hall International. Inc. London
Sobotta ?histologie? farbatlasder zytologie, Histologie und mikroskopischen anatomie des menschen/urban and schwarzenberg . Münih
 

Course outline weekly

WeeksTopics
Week 1The usage of microscopes, instruments and matters (T) Practice study (P)
Week 2The fixation of various cells and tissues of animals (T) Dissection of laboratory animals, taking of various tissue and fixation (P)
Week 3Many fixation materials (T) Passing of alchol series (P)
Week 4Sectioning (T) Embedding to paraffine (P)
Week 5General staining (T) Staining of Hematoxylen and eosin method (P)
Week 61st Midterm exam (T) Staining of Hematoxylen and eosin method (P)
Week 7Special staining (T) Staining of Azan method(P)
Week 8Immunohistochemistry (T) Immunohistochemical staining (P)
Week 9Prepara¬tion of museum materials. (T) Investigation of museum materials (P)
Week 10The special methods for the preservation of museum materials (T) Investigation of museum materials (P)
Week 11Cryıotomy (T) Sectioning by Cryıotomy (P)
Week 12EM techniques (T) Submission of preparations and discussions (P)
Week 132st Midterm exam (T) Submission of preparations and discussions (P)
Week 14EM techniques (T) Submission of preparations and discussions (P)
Week 15Studying for final exam
Week 16Final exam

Assesment methods

Course activitiesNumberPercentage
Attendance00
Laboratory130
Application00
Field activities00
Specific practical training00
Assignments00
Presentation110
Project00
Seminar00
Midterms220
Final exam140
Total100
Percentage of semester activities contributing grade succes060
Percentage of final exam contributing grade succes040
Total100

WORKLOAD AND ECTS CALCULATION

Activities Number Duration (hour) Total Work Load
Course Duration (x14) 14 1 14
Laboratory 13 4 52
Application000
Specific practical training000
Field activities000
Study Hours Out of Class (Preliminary work, reinforcement, ect)1210120
Presentation / Seminar Preparation155
Project000
Homework assignment21020
Midterms (Study duration)21530
Final Exam (Study duration) 12020
Total Workload4565261

Matrix Of The Course Learning Outcomes Versus Program Outcomes

D.9. Key Learning OutcomesContrubition level*
12345
1. Improves knowledge in proficiency level based on the undergraduate level in biology or different disciplines by using scientific methods to analyse and interpret it.     X
2. Uses theoretical and practical knowledge obtained from his/her field in proficiency level    X 
3. Interprets the knowledge obtained from his/her field with integrating the acquired knowledge from the other disciplines and synthesize new knowledge.    X
4. Gain ability to solve problems in his/her using research methods.     X
5. Gain ability to conduct study independently required in his/her field of specialization.     X
6. Improves new strategies to solve complex problems in the field of specialization.   X 
7. Uses acquired proficiency level knowledge and skills in processes of learning in his/her field.    X 
8. Uses computer software with computer technologies that is required in his/her field.    X 
9. Has the ability of minding social, scientific, cultural and ethical values in the levels of collecting, interpreting and applying the data in his/her field.     X
10. Evaluates the important events and cases by minding the results which take part in the development of his/her field.   X 

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest