BES627 - RESEARCH TECHNIQUES IN NUTRITIONAL SCIENCES
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| RESEARCH TECHNIQUES IN NUTRITIONAL SCIENCES | BES627 | 1st Semester | 1 | 3 | 2 | 7 |
| Prequisites | None | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Preparing and/or Presenting Reports Drill and Practice | |||||
| Instructor (s) | Assoc. Prof. Dr. Zeynep Göktaş | |||||
| Course objective | To learn different methods and techniques used in experimental evaluation of nutritional status in cell / human / animal samples and to develop skills related to the subject | |||||
| Learning outcomes |
| |||||
| Course Content | Principles of experimental design, techniques, laboratory practices and various assessment methods, animal models in nutritional sciences | |||||
| References | 1) Ninfa AJ, Ballou DP, Benore M. Fundamental Laboratory Approaches for Biochenistry and Biotechnology, Wiley, 2010. 2) Carson S. Molecular Biology Techniques (Third Edition), Elsevier, 2012. 3) Sharp, Patrick E. The Laboratory Rat, CRC Press, 2013. 4) Davis JM. Animal Cell Culture: Essential Methods, Wiley, 2011. 5) Heftmann E. Chromatography : fundamentals and applications of chromatography and related differential migration methods. Part A: Fundamentals and techniques, Part B: Applications, Elsevier, 2004. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Introduction to research techniques in nutritional sciences |
| Week 2 | Laboratory safety and research ethics |
| Week 3 | Basic laboratory techniques in nutritional sciences |
| Week 4 | RNA & DNA isolation techniques |
| Week 5 | Protein isolation techniques |
| Week 6 | PCR techniques-1 |
| Week 7 | PCR techniques-2 |
| Week 8 | Midterm |
| Week 9 | Total protein quantification methods |
| Week 10 | Protein analysis techniques-1 |
| Week 11 | Protein analysis techniques-2 |
| Week 12 | Chromatography methods in protein analysis |
| Week 13 | Cell culture techniques-1 |
| Week 14 | Cell culture techniques-2 |
| Week 15 | Preparation for Final Exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 0 | 0 |
| Laboratory | 0 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 1 | 20 |
| Presentation | 0 | 0 |
| Project | 1 | 20 |
| Seminar | 0 | 0 |
| Midterms | 1 | 20 |
| Final exam | 1 | 40 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 3 | 60 |
| Percentage of final exam contributing grade succes | 1 | 40 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 1 | 14 |
| Laboratory | 14 | 1 | 14 |
| Application | 14 | 2 | 28 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 3 | 42 |
| Presentation / Seminar Preparation | 0 | 0 | 0 |
| Project | 1 | 30 | 30 |
| Homework assignment | 5 | 3 | 15 |
| Midterms (Study duration) | 1 | 20 | 20 |
| Final Exam (Study duration) | 1 | 40 | 40 |
| Total Workload | 64 | 100 | 203 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Has up-to-date knowledge based on evidence in the field and is knowledgeable about technological tools. | X | ||||
| 2. Interprets, analyzes, and reports data from individual or interdisciplinary scientific studies. | X | ||||
| 3. Conducts scientific research using the technological equipment necessary to generate new knowledge based on the information acquired in the field. | X | ||||
| 4. Conducts synthesis and analysis of acquired information, interprets, reports findings. | X | ||||
| 5. Utilizes appropriate statistical methods for the analysis of scientific research. | X | ||||
| 6. Publishes and presents scientific articles independently or collaboratively on field-related topics. | X | ||||
| 7. Takes responsibility when faced with problems related to the field, sets an example for the community, proposes solutions, and utilizes communication technologies. | X | ||||
| 8. Embraces lifelong learning principles, keeps oneself updated, evaluates and interprets scientific articles with evidence-based practices. | X | ||||
| 9. Engages in national or international teamwork within the field, discusses and shares current scientific developments with individuals or groups outside the field through verbal or written communication when necessary. | X | ||||
| 10. Gathers and interprets scientific information ethically, contributes to, conducts, manages, and evaluates studies that will guide national and international nutrition plans and policies, prioritizing individuals and society. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest