BYL618 - APPLICATIONS of PLANT CYTOGENETICS
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| APPLICATIONS of PLANT CYTOGENETICS | BYL618 | 2nd Semester | 3 | 0 | 3 | 9 |
| Prequisites | ||||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Question and Answer Demonstration Experiment | |||||
| Instructor (s) | Assoc. Prof. Nihal Gömürgen | |||||
| Course objective | Recognition of plant chromosomes, methods for investigation of plant chromosomes, karyotpe analysis, meiosis analysis, Identification and assessment of genotoxicity with Allium test of chromosomal abnormalities | |||||
| Learning outcomes |
| |||||
| Course Content | Equipments and Chemicals for Chromosome Studies. Method of Measurements with Microscope, Taking The Photograph on Microscope and Microphotography, Measurement of Chromosomes on Microphotographs by a Computer Program. Preparation of Chemicals and Dyes Used. Method for to Study, Plant Somatic Chromosomes, to Obtain Materials, Pretreatments, Fixing, Hydrolize, Staining and to Obtain Permanent Preperations, Karyotype Analysis, Create Karyogram and Idiogram, Plant Somatic Chromosome Anomalies and Analysis, Evaluation of The Genotoxicity of Chemicals With Allium Test, Genotoxicity Tests are Accepted According to the OECD, Methods for The Study of Meiosis in Plant Chromosomes, Meiosis Abnormalities and Meiosis, Analysis. | |||||
| References | Darlington, C.D., La Cour, C.D., The Handling of Chromosomes, George Allen & Unwin Ltd. London, 263 pp. 1962. Elçi, Ş., Sitogenetikte Araştırma Yöntemleri ve Gözlemler, 100. Yıl Üniversitesi Yayınları No: 18, 238 pp. Van, 1994. Topaktaş, M., Rencüzoğulları, E., Sitogenetik, 182 pp. Adana 1995. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Introduction. History of cytogenetics General information about chromosomes and importance of chromosome observations (T) Observation of the chromosomes (mitosis phases) (P) |
| Week 2 | Chemicals and equipments for to research chromosomes (T) Observation of the chromosomes (meiosis phases) (P) |
| Week 3 | The ways of obtaining materials for observing chromosomes (T) Material supply for the observation of chromosomes (Obtaining root tips of onion & seeds) (P) |
| Week 4 | Tecniques of preparation of chemicals, solutions and stains which are used for making preparations of chromosomes (T) Preparation of the necessary chemicals & the solution and dyes (P) |
| Week 5 | Tecniques of cytogenetic preparations. Permanent preparations Microfotography of chromosomes by using microscope. Digital microfografie and saving them on computer (T) Continue to develop the necessary dyes and solutions (P) |
| Week 6 | Midterm exam I |
| Week 7 | Diffrent tecniques for to count and observe chromosomes on plants ,which are difficult to study (T) Pretreatments on root tips and stocking. Make preparations (P) |
| Week 8 | Chromosome microphotography with mikroskop. Use of the computer measurement program (T) Make preparations with different dyes. Observation of chromosomes. Make permanent preparatios, Microphotography on permanent preparations (P) |
| Week 9 | Karyotype analysis of chromosomes (length of chromosomes, relative lenght, chromosome index) Creation idiograms and karyograms (T) Measurement of the height of chromosomes. Measurement of chomossomes with computer programme (P) |
| Week 10 | Somatic chromosome abnormalities and their reasons. To create chromoseome abnormalities (T) Obtaining chromosome abnormalities with diffrent chemicals (P) |
| Week 11 | Allium test and evaluation method of expression (T) Observation of somatic chromosome aberrations (P) |
| Week 12 | Midterm exam II |
| Week 13 | Supply materal for the analysis of meiosis analysis. Tecnique of making meiosis preparations Tecnique of making meiosis preparations (T) Making and observing meiosis preparetions, Continue meiosis analysis, (P) |
| Week 14 | Meiosis analysis (T) Making permanent preparations for report and taking photos (P) |
| Week 15 | Studying for final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 0 | 0 |
| Laboratory | 1 | 30 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 0 | 0 |
| Presentation | 0 | 0 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 2 | 30 |
| Final exam | 1 | 40 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 3 | 60 |
| Percentage of final exam contributing grade succes | 1 | 40 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 14 | 3 | 42 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 10 | 140 |
| Presentation / Seminar Preparation | 0 | 0 | 0 |
| Project | 0 | 0 | 0 |
| Homework assignment | 0 | 0 | 0 |
| Midterms (Study duration) | 2 | 15 | 30 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 45 | 50 | 260 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Improves knowledge in proficiency level based on the undergraduate level in biology or different disciplines by using scientific methods to analyse and interpret it. | X | ||||
| 2. Uses theoretical and practical knowledge obtained from his/her field in proficiency level | X | ||||
| 3. Interprets the knowledge obtained from his/her field with integrating the acquired knowledge from the other disciplines and synthesize new knowledge. | X | ||||
| 4. Gain ability to solve problems in his/her using research methods. | X | ||||
| 5. Gain ability to conduct study independently required in his/her field of specialization. | X | ||||
| 6. Improves new strategies to solve complex problems in the field of specialization. | X | ||||
| 7. Uses acquired proficiency level knowledge and skills in processes of learning in his/her field. | X | ||||
| 8. Uses computer software with computer technologies that is required in his/her field. | X | ||||
| 9. Has the ability of minding social, scientific, cultural and ethical values in the levels of collecting, interpreting and applying the data in his/her field. | X | ||||
| 10. Evaluates the important events and cases by minding the results which take part in the development of his/her field. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest