BÄ°K619 - LAB WORK
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| LAB WORK | BÄ°K619 | Any Semester/Year | 1 | 2 | 2 | 3 |
| Prequisites | - | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Question and Answer Observation Team/Group Work Experiment Drill and Practice Problem Solving | |||||
| Instructor (s) | Prof. Dr. Yasemin Aksoy, Dr.Esra Büber | |||||
| Course objective | Aim of this course is to give theoretical and practical knowledge to the beginners of biochemistry about the chemicals, instruments and basic technics used in the biochemistry laboratories | |||||
| Learning outcomes |
| |||||
| Course Content | Practical studies of the biochemical laboratory | |||||
| References | Burtis CA, Ashwood ER, Bruns DE (ed) Tietz Fundamentals of Clinical Chemistry, Saunders Elsevier, St Louis, 2001 pp42-63 Henry JB Clinical Diagnosis and Management by Laboratory Methods, W.B: Saunders Co. Philadelphia, 2001 | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Introduction to the laboratories, research ethics |
| Week 2 | Laboratory safety: physical, chemical and biological |
| Week 3 | Water systems and chemicals: technical properties and storage |
| Week 4 | Properties of glass and plastic ware; cleaning of laboratory ware. |
| Week 5 | Measurement, units of measurement, significant figures, sources of measurement error, recording the laboratory data, presentation of the results; |
| Week 6 | Weighing, selection of the balance for the purpose; maintenance of the balances |
| Week 7 | Volumetric ware and their calibrations;, titrimetric procedures |
| Week 8 | Preparation and storage of the solutions:: w/w, w/v, v/v solutions |
| Week 9 | Preparation and storage of the solutions Molar, normal solutions, dilution |
| Week 10 | Buffer solutions, pH adjustment, usage and maintenance of pHmeters |
| Week 11 | Basic laboratory technics: filtration, concentration, lyophylization |
| Week 12 | usage and maintenance of centrifuges, selection of the centrifuge |
| Week 13 | Sample preparation: homogenization, preparation and storage of serum |
| Week 14 | Spektrophotometer: Lambert-Beer Law, calibration curve |
| Week 15 | Spectrophotometric protein measurement methods |
| Week 16 | Exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 15 | 20 |
| Laboratory | 0 | 0 |
| Application | 15 | 20 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 1 | 10 |
| Presentation | 0 | 0 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 0 | 0 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 31 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 0 | 0 | 0 |
| Laboratory | 15 | 2 | 30 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 15 | 2 | 30 |
| Presentation / Seminar Preparation | 0 | 0 | 0 |
| Project | 0 | 0 | 0 |
| Homework assignment | 1 | 10 | 10 |
| Midterms (Study duration) | 0 | 0 | 0 |
| Final Exam (Study duration) | 1 | 20 | 20 |
| Total Workload | 32 | 34 | 90 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. The student has detailed knowledge on biomolecules and metabolism; and applies and enhances this knowledge. | X | ||||
| 2. The student knows and uses the equipment and instruments specific to the field of biochemistry. | X | ||||
| 3. The student has fundamental laboratory knowledge such as laboratory safety and basic biochemical methods, and uses this information. | X | ||||
| 4. Knows basic statistical methods, applies this knowledge to study and interprets the results. | X | ||||
| 5. Knows professional ethics (intellectual property, academic integrity, ethics in human relations: avoids discrimination, values merit, respects privacy etc), research and publication ethics and applies this information. | X | ||||
| 6. Can access printed and electronic databases in the field and has the capacity to follow up recent advances in biochemistry and related fields. | X | ||||
| 7. Comprehends the interaction of biochemistry with other disciplines and makes use of them. | X | ||||
| 8. By means of theoretical knowledge and critical reasoning, plans the related experiments and puts into practice the relevant experimental approaches to his/her special field of study. | X | ||||
| 9. Evaluates the results of the study, interprets the data and writes its report. | X | ||||
| 10. Independently or with the help of an advisor can define a scientific or technical problem, propose a solution and if needed solves the problem. | X | ||||
| 11. Takes responsibility in collaborative studies, contributes to the studies and works in harmony. | X | ||||
| 12. Makes a presentation on a recent topic in the field of biochemistry. | X | ||||
| 13. Presents or publishes his/her studies in a scientific meeting or a journal. | X | ||||
| 14. Reads, comprehends, interprets and criticizes an article in the field of biochemistry. | X | ||||
| 15. Has proficiency in English required to follow the advances in biochemistry. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest