EAT710 - BIOANALYSIS METHODS II
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| BIOANALYSIS METHODS II | EAT710 | Any Semester/Year | 3 | 3 | 4 | 9 |
| Prequisites | ||||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion | |||||
| Instructor (s) | Prof.Dr.Ülkü Ündeğer Bucurgat, Prof.Dr.Nurşen Başaran, Prof.Dr.Terken Baydar, Doç.Dr.Aylin Gürbay, Doç.Dr. Pınar Erkekoğlu, Doç.Dr. Gözde Girgin, Doç.Dr. Sevtap Aydın Dilsiz, Doç.Dr Suna Sabuncuoğlu | |||||
| Course objective | Methods used in analytical toxicology, instrumental analysis, identification and determination methods of toxic compounds including drugs in biological samples | |||||
| Learning outcomes |
| |||||
| Course Content | Chromatographic methods used for the analysis of toxic substances in biological materials, gas chromatography, high performance liquid chromatography, atomic absorption spectroscopy and other modern instrumental analytical methods | |||||
| References | 1. Hayes, A.W., "Principles and Methods of Toxicology", Taylor & Francis, Philadelphia, 2001. 2. Hicks, M.R., Haven, M.C., Schenken, J.R., McWhoter, C.A., Laboratory Instrumentation, Philadelphia, 1988. 3. Ballantyne, B., Mars, T., Syversen, T., General and Applied Toxicology, New York, 2000. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Atomic Absorption Spectroscopy |
| Week 2 | Atomic Absorption Spectroscopy |
| Week 3 | Isolation of microsome and lymphocyte |
| Week 4 | Gas Chromatography |
| Week 5 | Gas Chromatography |
| Week 6 | Gas Chromatography- Mass Spectroscopy |
| Week 7 | High Performance Liquid Cromatography |
| Week 8 | High Performance Liquid Cromatography |
| Week 9 | High Performance Liquid Cromatography |
| Week 10 | Basic principles of enzyme measurements and computations in toxicologic analysis |
| Week 11 | Spectroscopy |
| Week 12 | Spectroscopy |
| Week 13 | 3R in toxicological analysis |
| Week 14 | Methods of measurements in cytotoxicity |
| Week 15 | Genotoxicity tests |
| Week 16 | Final |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 0 | 0 |
| Laboratory | 0 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 2 | 30 |
| Presentation | 0 | 0 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 0 | 0 |
| Final exam | 1 | 70 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 0 | 30 |
| Percentage of final exam contributing grade succes | 0 | 70 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 3 | 42 |
| Laboratory | 0 | 0 | 0 |
| Application | 14 | 3 | 42 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 4 | 56 |
| Presentation / Seminar Preparation | 0 | 0 | 0 |
| Project | 0 | 0 | 0 |
| Homework assignment | 2 | 50 | 100 |
| Midterms (Study duration) | 0 | 0 | 0 |
| Final Exam (Study duration) | 1 | 30 | 30 |
| Total Workload | 45 | 90 | 270 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. They have advanced and updated knowledge about the possible toxic effects of chemical, physical and biological agents. | X | ||||
| 2. They have strong information on different fields of toxicology and can integrate with different science disciplines with systematic approach. | X | ||||
| 3. They can conduct studies according to ethical principles, independently plan and carry out a project in the field of toxicology, perform advanced toxicological analyses with many different analytical techniques and evaluate the results with appropriate statistical analyses. | X | ||||
| 4. They can efficiently communicate with professional toxicologists; carry out teamwork and collaborate with national and international scientists. | |||||
| 5. They know different information technologies and can perform toxicoinformatic studies. | X | ||||
| 6. They can evaluate and interpret the information obtained from these analyses, elucidate different molecular pathways, discuss the results and present them in national and/or international meetings along with publishing their data in high impact journals. | X | ||||
| 7. They have information on the effects of toxic agents and pharmaceuticals in susceptible populations and different pathological conditions and on the diagnosis and the treatment of intoxications. | X | ||||
| 8. They are knowledgeable about evaluating the effects of environmental pollutants and toxic substances, nutritional and herbal supplements and food on public health and informing the public as well as the regulatory authorities about this issue. | |||||
| 9. They can contribute to the risk assessment and strategic decision-making processes and find the solutions for the toxicological problems encountered in daily life. | |||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest