BYF606 - BASIC CELL ELECTROPHYSIOLOGY

Course Name Code Semester Theory
(hours/week)		 Application
(hours/week)		 Credit ECTS
BASIC CELL ELECTROPHYSIOLOGY BYF606 2nd Semester 2 2 3 10
PrequisitesNone
Course languageTurkish
Course typeElective 
Mode of DeliveryFace-to-Face 
Learning and teaching strategies 
Instructor (s)Prof. Dr. Nuhan Puralı 
Course objectivePrinciple goal of the course is to supply the students with some fundamental information and initial experience in the electrophysiological methods and techniques, widely used in investigation of the excitable cells. 
Learning outcomes
  1. manufacturing various types of recording electrodes.
  2. preparation of brain slice, hart, cell culture and dissected neuron preparations
  3. performing some extracellular and intracellular recordings.
  4. performing some initial voltage and patch clamp experiments
  5. preparing the recordings for analysis and presentation
Course ContentNervous system is consisted of neurons and support cells. All those cells communicate with each other by using the electrical voltage signals developing across the cellular membrane. All the known neural functions are either directly or indirectly related to the bio-electrical signaling. Thus, recording of the electrical events under laboratory conditions is an essential and very common research method. However, as in all the cellular events bio-electrical signals are very minute. Reliable recording can be achieved only under properly controlled laboratory conditions and by using the most appropriate laboratory equipments. Through the course biological and physical basis of the bio-electrical signals will be considered firstly... 
ReferencesHücre elektrofizyolojisi ve Görüntülemenin Temelleri. Nuhan Puralı, Veri Medikal Ä°stanbul 2008. 

Course outline weekly

WeeksTopics
Week 1Electrical events across the cell membrane
Week 2Ion channels, ion exchangers and co-transporters.
Week 3Neuronal and non neuronal excitable cell preparations
Week 4Electrodes
Week 5Analog cell models
Week 6Extracellular recording methods
Week 7Recording the membran potential, the current clamp technique
Week 8Voltage clamp technique
Week 9Voltage clamp technique
Week 10Voltage clamp technique
Week 11Patch-clamp technique
Week 12Noise in electrophysiological data, data analysis, and digital modeling
Week 13How to present electrophysiological data
Week 14Essential principles when constructing a laboratory
Week 15Exam Preparation
Week 16Final Exam

Assesment methods

Course activitiesNumberPercentage
Attendance1410
Laboratory00
Application1425
Field activities00
Specific practical training00
Assignments115
Presentation00
Project00
Seminar00
Midterms00
Final exam150
Total100
Percentage of semester activities contributing grade succes2950
Percentage of final exam contributing grade succes150
Total100

WORKLOAD AND ECTS CALCULATION

Activities Number Duration (hour) Total Work Load
Course Duration (x14) 14 2 28
Laboratory 0 0 0
Application14228
Specific practical training000
Field activities000
Study Hours Out of Class (Preliminary work, reinforcement, ect)1410140
Presentation / Seminar Preparation000
Project000
Homework assignment13030
Midterms (Study duration)000
Final Exam (Study duration) 13030
Total Workload4474256

Matrix Of The Course Learning Outcomes Versus Program Outcomes

D.9. Key Learning OutcomesContrubition level*
12345
1. Graduates have knowledge related to the biophysical principles underlying all processes of life at the level of cell/tissue/organ/system    X
2. Has an ability use his/her higher intellectual processes such as critical thinking, problem solving decision development during his/her education periodX    
3. Can take part in some research activities to contribute to the solution of a problem in the field of biophysics    X
4. Awaring of the fact that biophysics is a multidisciplinary field, follows the developments in other branches of the Medical&Basic SciencesX    
5. Can use computer software and laboratory equipment to produce appropriate stimulus, acquire the biological signals under the ideal conditions, quantitatively analyse the raw data    X
6. Acquired knowledge at an expertise level in statistical methods. Can choose the most suitable method for his/her researchX    
7. Is aware of the importance of the ethical rules and regulations and perform laboratory research as defined by the GLP, Bio-Safety principlesX    
8. Has the capacity of successfully preparing and presenting the report of the research work he/she takes part in, publishing at least one manuscriptX    
9. Follows the activities of the national&international organizations related to his/her expertise and takes part in themX    
10. Shares the knowledge he/she acquired from biophysics with partners from all parts of the society; contributes to the formation of the knowledge-based societyX    

*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest