ESF608 - LABORATORY METHODS IN MUSCLE PHYSIOLOGY II
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| LABORATORY METHODS IN MUSCLE PHYSIOLOGY II | ESF608 | Any Semester/Year | 2 | 2 | 4 | 10 |
| Prequisites | None | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Experiment Project Design/Management | |||||
| Instructor (s) | Senay Akin, Ph.D. | |||||
| Course objective | This course is designed to give student, who works on muscle physiology, some laboratory techniques of muscle physiology, biochemistry and molecular biology before starting on working her/his own project. | |||||
| Learning outcomes |
| |||||
| Course Content | This course consists of both lectures and laboratory application. Basically, lectures will focus on therotical bacgrounds for the laboratory sections. Skeletal and cardiac muscle phenotype, stress response to skeletal muscle such as acute and chronic exercises, heat stress, hypoxia will be discussed. In that respect, both SDS poliacrylamide and native tube gel electrophoresis, western and immunohistochemical analysis of stress markers, antioxidant and anaerobic/aerobic enzyme activities along with the levels of oxidative muscle damage marker will be analyzed in the laboratory. | |||||
| References | There will be no suggested textbook. On the other hand, methods parts of various papers will be used as a course material. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | General principles of SDS gel electrophoresis |
| Week 2 | Prepareation of muscle samples for SDS gel electrophoresis |
| Week 3 | Isolation of skeletal muscle MHC?s by using SDS gel electrophoresis. |
| Week 4 | Isolation of skeletal muscle MLC?sby using SDS gel electrophoresis |
| Week 5 | Isolation of cardiac muscle myosin by using native gel electrophoresis |
| Week 6 | Assessment of muscle phenotype |
| Week 7 | Various stress models: Exercise training, hypoxia, heat |
| Week 8 | Immunohistochemistry |
| Week 9 | Western analysis |
| Week 10 | Western analysis |
| Week 11 | Measurement of citrate synthase enzyme activity |
| Week 12 | Measurement of myofibrillar ATPase activity |
| Week 13 | Measurement of SOD/GPx activities |
| Week 14 | Measurement of protein carbonyl in muscle tissue; Measurement of lipid peroxidation in muscle tissue |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | ||
| Laboratory | ||
| Application | ||
| Field activities | ||
| Specific practical training | ||
| Assignments | ||
| Presentation | ||
| Project | ||
| Seminar | ||
| Midterms | ||
| Final exam | ||
| Total | ||
| Percentage of semester activities contributing grade succes | ||
| Percentage of final exam contributing grade succes | ||
| Total | ||
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 0 | ||
| Laboratory | 0 | ||
| Application | 0 | ||
| Specific practical training | 0 | ||
| Field activities | 0 | ||
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 0 | ||
| Presentation / Seminar Preparation | 0 | ||
| Project | 0 | ||
| Homework assignment | 0 | ||
| Midterms (Study duration) | 0 | ||
| Final Exam (Study duration) | 0 | ||
| Total Workload | 0 | 0 | 0 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Students will be able to follow the conceptual and scientific developments related to basic measurement and evaluation techniques specific to the Exercise and Sports Physiology, and use the deep and systematic knowledge specific to the field of Exercise and Sports Physiology in their professional and academic studies. | X | ||||
| 2. Has the laboratory safety and technical knowledge and skills required for laboratory studies in Exercise and Sport Physiology, and able to solve the research problems using the tools and equipment in the field. | X | ||||
| 3. Has the competency to analyse and integrate the information from other disciplines, synthesize new information, make decisions and solve problems by using theoretical and applied concepts and principles in Exercise and Sport Physiology with a critical approach. | X | ||||
| 4. Has the competency to conduct research, take part in research projects, chose the appropriate statistical methods, interpret the results of his/her work, write the report and present it in scientific meetings or publish. | X | ||||
| 5. Evaluates national/international strategies, politics and practices related to Exercise and Sports Physiology in line with the national values and country realities. | X | ||||
| 6. Acts in compliance with legislation, professional values and ethical principles in the formation of knowledge related to Exercise and Sports Physiology, and transfers his/her professional knowledge, skills and responsibilities to team work. | X | ||||
| 7. Reads and analyses the scientific papers in Exercise and Sports Physiology, conducts and carries out evidenced based research in the concerned professional field. | X | ||||
| 8. Knows the importance of communication with other sciences related to Exercise and Sport Physiology, uses his/her knowledge and problem solving abilities in interdisciplinary studies. | X | ||||
| 9. Adopts lifelong learning principles, identifies his/her learning needs and goals, and contributes to quality improvement, vocational training and promotion programs in Exercise and Sports Physiology. | X | ||||
| 10. Establishes written, verbal and visual communication with national and international scientists and defends his/her opinions on the issues in Exercise and Sports Physiology. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest