ESF607 - LABORATORY METHODS IN MUSCLE PHYSIOLOGY I
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| LABORATORY METHODS IN MUSCLE PHYSIOLOGY I | ESF607 | Any Semester/Year | 2 | 2 | 4 | 10 |
| Prequisites | None | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Experiment Project Design/Management | |||||
| Instructor (s) | Senay Akin, Ph.D. | |||||
| Course objective | This course is designed to give student, who works on muscle physiology, basic principles and the methodology of muscle physiology and biochemistry laboratory techniques before starting on working her/his own project. | |||||
| Learning outcomes |
| |||||
| Course Content | This course consists of both lectures and laboratory application. Basically, lectures will focus on therotical bacgrounds for the laboratory sections. In that respect, skeletal muscle structure, localization and characteristics, histochemically fiber typing, acut and chronic exercise tranining models via tradmill running and swimming, muscle atrophy models, preperation of basic chemical solutions and various methods to measure muscle protein content will be discussed and applied in the laboratory. | |||||
| References | There will be no suggested textbook. On the other hand, methods parts of various papers will be used as a course material. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Exercise training: Treadmill running |
| Week 2 | |
| Week 3 | Muscle atrophy models: Hindlimb suspension |
| Week 4 | Macroscopic apperance of skeletal muscles: White and Red muscles and their localizations |
| Week 5 | How to identify and isolate various locomotor muscles and left and right ventricle |
| Week 6 | Preperation of muscle sections for both histochemistry and biochemical measurement |
| Week 7 | Histochemistry for fiber typing: Cutting muscle sections by using cryostat |
| Week 8 | Preparing solutions for ATPase histochemistry for skeletal muscle fiber typing |
| Week 9 | Fiber type determination, measuring CSA, assessment of fiber type distribution |
| Week 10 | Paper discussion related to histochemical fiber typing |
| Week 11 | Preperations of various biochemical solutions, homogenization of muscle tissue |
| Week 12 | Isolation of myofibrillar and connective tissue protein |
| Week 13 | How to determine muscle protein content: Biuret, Bradford and Lowry methods |
| Week 14 | Measurement of myofibrillar and connective tissue protein |
| Week 15 | Preparation to final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | ||
| Laboratory | ||
| Application | ||
| Field activities | ||
| Specific practical training | ||
| Assignments | ||
| Presentation | ||
| Project | ||
| Seminar | ||
| Midterms | ||
| Final exam | ||
| Total | ||
| Percentage of semester activities contributing grade succes | ||
| Percentage of final exam contributing grade succes | ||
| Total | ||
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 0 | ||
| Laboratory | 0 | ||
| Application | 0 | ||
| Specific practical training | 0 | ||
| Field activities | 0 | ||
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 0 | ||
| Presentation / Seminar Preparation | 0 | ||
| Project | 0 | ||
| Homework assignment | 0 | ||
| Midterms (Study duration) | 0 | ||
| Final Exam (Study duration) | 0 | ||
| Total Workload | 0 | 0 | 0 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Students will be able to follow the conceptual and scientific developments related to basic measurement and evaluation techniques specific to the Exercise and Sports Physiology, and use the deep and systematic knowledge specific to the field of Exercise and Sports Physiology in their professional and academic studies. | X | ||||
| 2. Has the laboratory safety and technical knowledge and skills required for laboratory studies in Exercise and Sport Physiology, and able to solve the research problems using the tools and equipment in the field. | X | ||||
| 3. Has the competency to analyse and integrate the information from other disciplines, synthesize new information, make decisions and solve problems by using theoretical and applied concepts and principles in Exercise and Sport Physiology with a critical approach. | X | ||||
| 4. Has the competency to conduct research, take part in research projects, chose the appropriate statistical methods, interpret the results of his/her work, write the report and present it in scientific meetings or publish. | X | ||||
| 5. Evaluates national/international strategies, politics and practices related to Exercise and Sports Physiology in line with the national values and country realities. | X | ||||
| 6. Acts in compliance with legislation, professional values and ethical principles in the formation of knowledge related to Exercise and Sports Physiology, and transfers his/her professional knowledge, skills and responsibilities to team work. | X | ||||
| 7. Reads and analyses the scientific papers in Exercise and Sports Physiology, conducts and carries out evidenced based research in the concerned professional field. | X | ||||
| 8. Knows the importance of communication with other sciences related to Exercise and Sport Physiology, uses his/her knowledge and problem solving abilities in interdisciplinary studies. | X | ||||
| 9. Adopts lifelong learning principles, identifies his/her learning needs and goals, and contributes to quality improvement, vocational training and promotion programs in Exercise and Sports Physiology. | X | ||||
| 10. Establishes written, verbal and visual communication with national and international scientists and defends his/her opinions on the issues in Exercise and Sports Physiology. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest