KBÄ°701 - LABORATUARY TECNIQUES IN BASIC ONCOLOGY
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| LABORATUARY TECNIQUES IN BASIC ONCOLOGY | KBÄ°701 | 2nd Semester | 2 | 2 | 3 | 8 |
| Prequisites | None | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Question and Answer Preparing and/or Presenting Reports Drill and Practice | |||||
| Instructor (s) | Prof. Dr. Güneş Esendağlı, Doç. Dr. Hande Canpınar, Doç.Dr. Gürcan Günaydın, Doç.Dr. Begüm Kocatürk | |||||
| Course objective | The main purpose of this course to make the students understand and apply the laboratory techniques and methods that are commonly used in research activities in the field of basic oncology. The in vivo and in vitro models employed in basic cancer research as well as principles of the immunological and molecular techniques used in cancer studies are described. Safety rules in the laboratories are emphasized. Lectures are mainly theoretical, however the teaching of basic laboratory techniques are supported with practical sessions. Each student is responsible for reading the selected literature and its relevant references suggested for each lecture, preparing one seminar presentation on a selected topic and submitting the report of his/her seminar. | |||||
| Learning outcomes |
| |||||
| Course Content | Laboratory safety, aseptic technique and cell cultures, analyses of drug resistance, monoclonal antibody technology and immunological techniques, advanced molecular techniques in oncology, molecular cloning, gene delivery and in vivo tumor models. | |||||
| References | 1. Freshney R.I., Freshney M.G. (2002). Culture of specialized cells. Wiley-Liss, Inc., New York. 2. Old, R.W., Primrose, S.B. (1998). Principles of Gene Manipulation, 5. baskı, Blackwell Science Ltd, Oxford. 3. Sambrook, J., Fritsch, E. F., Maniatis, T. (2005). Molecular clonning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor. | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Laboratory Safety |
| Week 2 | Solution Preparation and Calculation |
| Week 3 | Laboratory Practice (Solution Preparation ) |
| Week 4 | Cell Culture (I) |
| Week 5 | Cell Culture (II) |
| Week 6 | DNA/RNA Isolation and Quality Determination |
| Week 7 | PCR and PCR-related Techniques |
| Week 8 | Laboratory Practice (PCR/QPCR) |
| Week 9 | High-throughput Technologies |
| Week 10 | Molecular Cloning |
| Week 11 | DNA Squence Analyzes |
| Week 12 | Gene Delivery Techniques |
| Week 13 | Microscope and Immunohistochemistry |
| Week 14 | Laboratory Practice (Cytospin/ Cell Staining) |
| Week 15 | General Exam Preparation |
| Week 16 | Final Exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 14 | 10 |
| Laboratory | 0 | 0 |
| Application | 3 | 10 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 1 | 40 |
| Presentation | 1 | 40 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 0 | 0 |
| Final exam | 0 | 0 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 17 | 20 |
| Percentage of final exam contributing grade succes | 2 | 80 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 0 | 0 | 0 |
| Application | 3 | 2 | 6 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 11 | 154 |
| Presentation / Seminar Preparation | 1 | 11 | 11 |
| Project | 1 | 14 | 14 |
| Homework assignment | 1 | 35 | 35 |
| Midterms (Study duration) | 0 | 0 | 0 |
| Final Exam (Study duration) | 1 | 30 | 30 |
| Total Workload | 35 | 105 | 278 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Produces educational materials that shape the clinical applications or academic studies, or applies the novel methods into a new area on molecular, cellular and systemic effects of surgery on body physiology, cell biology, wound healing, immunology, disease pathophysiology and genetic mechanisms. | X | ||||
| 2. Analyzes and reports the data from his/her research by choosing appropriate statistical methods. Be aware of the importance of statistical and biological significance, data stability (or consistency) and heterogeneity on results. | X | ||||
| 3. Uses knowledge on clinical and academic applications and state of the art technology products. Transfers knowledge that comes from the research results into his/her applications. | X | ||||
| 4. Plans and maintains his/her research independently and according to the ethical guidelines, develops a knowledge that is needed to take part in national and international projects. Performs life long learning principles. | X | ||||
| 5. Uses the know-how about surgical research and basic science for benefit of the society, makes contributions to the national and international policies for disease prevention and treatment with other disciplines. Takes part in the updating, developing and applying the surgical research study programs, works as a consultant, who has knowledge and skills about the topic. | X | ||||
| 6. Being able to plan, apply and distribute the roles as a director or leader in multidisciplinary academic or scientific societies; has an ability to solve the conflicts. By using at least one foreign language, communicate equally in written, oral and visually in academic and professional environments. | X | ||||
| 7. Shows a multidisciplinary approach to surgical and basic science studies. Understands the health sciences on different areas, makes connections between them and presents novel scientific approaches and solutions to hypotheses. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest