BYL670 - ADVANCED BIOCHEMICAL TECHNIQUES
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| ADVANCED BIOCHEMICAL TECHNIQUES | BYL670 | 1st Semester | 2 | 3 | 3 | 9 |
| Prequisites | ||||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Discussion Question and Answer Demonstration Experiment | |||||
| Instructor (s) | Assoc. Prof. Işık Perçin Demirçelik | |||||
| Course objective | To teach the basic working principle of instruments which are frequently used in biochemical studies. | |||||
| Learning outcomes |
| |||||
| Course Content | Spectrophotometric methods and usage of spectrophotometer Centrifugation methods and usage of centrifuge Electrophoresis methods and applications Gel imaging systems Chromatographic methods Polymerase chain reaction and DNA sequence analysis Magnetic resonance and refractometer Electron spin resonance | |||||
| References | ||||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Spectrophotometric methods (T) Usage of spectrophotometer (P) |
| Week 2 | Centrifugation methods (T) Usage of centrifuge (P) |
| Week 3 | Electrophoretic methods (T) Application of agarose gel electrophoresis (P) |
| Week 4 | Electrophoretic methods (T) Polyacrylamide gel electrophoresis application (P) |
| Week 5 | Presentation |
| Week 6 | Two dimensional gel electrophoresis (T) Two dimensional gel electrophoresis application (P) |
| Week 7 | Gel imaging systems (T) Usage of gel imaging systems (P) |
| Week 8 | Chromatographic methods (T) Affinity chromatography application (P) |
| Week 9 | Midterm |
| Week 10 | Polymerase chain reaction (T) Amplification of a specific DNA region (P) |
| Week 11 | DNA sequence analysis (T) Sequence analysis of a specific DNA sequence |
| Week 12 | Magnetic resonance and refractometer Applications of magnetic resonance and refractometer (P) |
| Week 13 | Electron spin resonance (T) Application of electron spin resonance (P) |
| Week 14 | Presentation |
| Week 15 | Studying for final exam |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 0 | 0 |
| Laboratory | 0 | 0 |
| Application | 0 | 0 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 0 | 0 |
| Presentation | 2 | 40 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 1 | 20 |
| Final exam | 1 | 40 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 0 | 60 |
| Percentage of final exam contributing grade succes | 0 | 40 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 2 | 28 |
| Laboratory | 14 | 3 | 42 |
| Application | 0 | 0 | 0 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 12 | 10 | 120 |
| Presentation / Seminar Preparation | 2 | 15 | 30 |
| Project | 0 | 0 | 0 |
| Homework assignment | 0 | 0 | 0 |
| Midterms (Study duration) | 1 | 15 | 15 |
| Final Exam (Study duration) | 1 | 30 | 30 |
| Total Workload | 44 | 75 | 265 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Improves knowledge in proficiency level based on the undergraduate level in biology or different disciplines by using scientific methods to analyse and interpret it. | X | ||||
| 2. Uses theoretical and practical knowledge obtained from his/her field in proficiency level | X | ||||
| 3. Interprets the knowledge obtained from his/her field with integrating the acquired knowledge from the other disciplines and synthesize new knowledge. | X | ||||
| 4. Gain ability to solve problems in his/her using research methods. | X | ||||
| 5. Gain ability to conduct study independently required in his/her field of specialization. | X | ||||
| 6. Improves new strategies to solve complex problems in the field of specialization. | X | ||||
| 7. Uses acquired proficiency level knowledge and skills in processes of learning in his/her field. | X | ||||
| 8. Uses computer software with computer technologies that is required in his/her field. | X | ||||
| 9. Has the ability of minding social, scientific, cultural and ethical values in the levels of collecting, interpreting and applying the data in his/her field. | X | ||||
| 10. Evaluates the important events and cases by minding the results which take part in the development of his/her field. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest