KÄ°M706 - BIOMOLECULAR COMPLEXES and MOLECULAR RECOGNITION
| Course Name | Code | Semester | Theory (hours/week) |
Application (hours/week) |
Credit | ECTS |
|---|---|---|---|---|---|---|
| BIOMOLECULAR COMPLEXES and MOLECULAR RECOGNITION | KÄ°M706 | Any Semester/Year | 3 | 0 | 3 | 10 |
| Prequisites | none | |||||
| Course language | Turkish | |||||
| Course type | Elective | |||||
| Mode of Delivery | Face-to-Face | |||||
| Learning and teaching strategies | Lecture Question and Answer Preparing and/or Presenting Reports Demonstration Drill and Practice | |||||
| Instructor (s) | Assoc. Prof. Dr. Mehmet Atakay | |||||
| Course objective | The purpose of this course is to inform the students about non-covalent interactions, protein protein interactions, interactions between proteins and other species such as metals, DNA, molecular imprinting, antigen-antibody interactions, proteomics, phosphoproteomics, aptamer, aptamer-protein interactions, mıcroarrays in protein interactions analysis, characterization of interactions between protein and DNA structures by using varied spectroscopic techniques. | |||||
| Learning outcomes |
| |||||
| Course Content | Protein-protein interactions, peptide-metal ion interactions, peptide-dye interactions, peptide/protein-enzyme interactions, peptide/protein-oligonuclueotide/DNA interactions, antigen-antibody complexes, receptor-ligand interactions, amino acid/peptide /protein-cyclodextrin interactions, aptamers, proteomics, analysis of enzymatic digestion fragments of proteins, amino acid sequence analysis of peptides, characterization of supramolecular complexes of biomolecules, carbohydrate analysis. | |||||
| References | Pavel Hobza P., Müller-Dethlefs K., Non-Covalent Interactions, Theory and Experiment, RSC Theoritical and Computational Chemistry Series, (2009) Komiyama M., Takeuchi T., Mukawa T., Asanuma H., Molecular Imprinting, Wiley, (2003) Downard K., Mass Spectrometry: A Foundation Course, Royal Society of Chemistry, (2004) Golemis E., Protein-Protein Interactions: A Molecular Cloning Manual, cold spring harbor laboratory press, (2001) Delaage M,. Molecular Recognition Mechanisms, Wiley, (1991) | |||||
Course outline weekly
| Weeks | Topics |
|---|---|
| Week 1 | Non-Covalent interactions |
| Week 2 | Molecular recognition strategies |
| Week 3 | Protein-Protein interactions |
| Week 4 | Protein-DNA, Protein-Dye, Protein-Metal interactions |
| Week 5 | Antigen-Antibody complexes |
| Week 6 | Molecular imprinted polymers |
| Week 7 | Proteomics studies |
| Week 8 | Biological mass spectrometry |
| Week 9 | Enzymatic digestion, amino acid sequence analysis |
| Week 10 | Midterm Exam |
| Week 11 | Phosphoproteomics studies |
| Week 12 | Aptamers and Aptamer-Protein interactions |
| Week 13 | Microarray applications |
| Week 14 | Characterization of supramolecular complexes of biomolecules |
| Week 15 | Drill and Practice |
| Week 16 | Final exam |
Assesment methods
| Course activities | Number | Percentage |
|---|---|---|
| Attendance | 0 | 0 |
| Laboratory | 0 | 0 |
| Application | 2 | 5 |
| Field activities | 0 | 0 |
| Specific practical training | 0 | 0 |
| Assignments | 10 | 25 |
| Presentation | 0 | 0 |
| Project | 0 | 0 |
| Seminar | 0 | 0 |
| Midterms | 1 | 20 |
| Final exam | 1 | 50 |
| Total | 100 | |
| Percentage of semester activities contributing grade succes | 13 | 50 |
| Percentage of final exam contributing grade succes | 1 | 50 |
| Total | 100 | |
WORKLOAD AND ECTS CALCULATION
| Activities | Number | Duration (hour) | Total Work Load |
|---|---|---|---|
| Course Duration (x14) | 14 | 3 | 42 |
| Laboratory | 0 | 0 | 0 |
| Application | 2 | 8 | 16 |
| Specific practical training | 0 | 0 | 0 |
| Field activities | 0 | 0 | 0 |
| Study Hours Out of Class (Preliminary work, reinforcement, ect) | 14 | 6 | 84 |
| Presentation / Seminar Preparation | 0 | 0 | 0 |
| Project | 0 | 0 | 0 |
| Homework assignment | 15 | 7 | 105 |
| Midterms (Study duration) | 1 | 20 | 20 |
| Final Exam (Study duration) | 1 | 40 | 40 |
| Total Workload | 47 | 84 | 307 |
Matrix Of The Course Learning Outcomes Versus Program Outcomes
| D.9. Key Learning Outcomes | Contrubition level* | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| 1. Specializes through postgraduate studies and introduces innovative scientific concepts to their field. | X | ||||
| 2. Analyzes sophisticated ideas and obtains original results by evaluating interdisciplinary interactions. | X | ||||
| 3. Evaluates, criticizes, interprets, and communicates new information in their field without prejudice. | X | ||||
| 4. Develops new ideas, methods, and applications in their field or adapts them to different areas. | X | ||||
| 5. Develops scientific strategies using various research methods based on advanced knowledge and experience. | X | ||||
| 6. Contributes to scientific knowledge by publishing original articles in national/international refereed journals. | X | ||||
| 7. Contributes to original work and interdisciplinary problem-solving, taking a leadership role in their field. | X | ||||
| 8. Develops new thoughts and methods using creative and critical thinking for problem-solving. | X | ||||
| 9. Defends original views and communicates effectively while discussing with competent people. | X | ||||
| 10. Accesses international sources, updates knowledge, and communicates with colleagues using a foreign language. | X | ||||
| 11. Conducts research in national and international scientific research groups. | X | ||||
| 12. Keeps track of advancements in their field, internalizes them, and contributes to society's knowledge and sustainability. | X | ||||
| 13. Manages data related to their field effectively and securely, considering societal, scientific, cultural, and ethical values. | X | ||||
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest